Embryonic day (E) 15.5 timed-pregnant female C57BL/6 and CD1 (Charles River; for Fig. 3 , Supplementary Figs. 20–22, 24, and 25 ) or Swiss Webster (Taconic; Supplementary Fig. 7, 23 ) mice were deeply anesthetized with 2% isoflurane. Uterine horns were exposed and periodically rinsed with warm sterile PBS. A plasmid encoding Archon1, Archon2 or miRFP (pCAG-Archon1/2-KGC-EGFP-ER2-WPRE, pAAV-Syn-miRFP; 1μg/μl) diluted with PBS was injected into the lateral ventricle of one cerebral hemisphere of an embryo. Five voltage pulses (50 V, 50 ms duration, 1 Hz) were delivered using round plate electrodes (CUY21 electroporator, NEPA GENE, Japan; ECM™ 830 electroporator, Harvard Apparatus). Injected embryos were placed back into the dam, and allowed to mature to delivery. All experimental manipulations were performed in accordance with protocols approved by the Harvard Standing Committee on Animal Care or Massachusetts Institute of Technology Committee on Animal Care (according to location of the respective experiments), following guidelines described in the US National Institutes of Health Guide for the Care and Use of Laboratory Animals.
C57bl 6 and cd1
Manufactured by Taconic Biosciences
C57BL/6 and CD1 are two common mouse strains used in biomedical research. C57BL/6 is an inbred strain, while CD1 is an outbred strain. Both strains are widely used as models for a variety of research applications.
Automatically generated - may contain errors
2 protocols using c57bl 6 and cd1
1
In Utero Electroporation of Fluorescent Probes
2
In Utero Electroporation of Fluorescent Probes
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Embryonic day (E) 15.5 timed-pregnant female C57BL/6 and CD1 (Charles River; for Fig. 3 , Supplementary Figs. 20–22, 24, and 25 ) or Swiss Webster (Taconic; Supplementary Fig. 7, 23 ) mice were deeply anesthetized with 2% isoflurane. Uterine horns were exposed and periodically rinsed with warm sterile PBS. A plasmid encoding Archon1, Archon2 or miRFP (pCAG-Archon1/2-KGC-EGFP-ER2-WPRE, pAAV-Syn-miRFP; 1μg/μl) diluted with PBS was injected into the lateral ventricle of one cerebral hemisphere of an embryo. Five voltage pulses (50 V, 50 ms duration, 1 Hz) were delivered using round plate electrodes (CUY21 electroporator, NEPA GENE, Japan; ECM™ 830 electroporator, Harvard Apparatus). Injected embryos were placed back into the dam, and allowed to mature to delivery. All experimental manipulations were performed in accordance with protocols approved by the Harvard Standing Committee on Animal Care or Massachusetts Institute of Technology Committee on Animal Care (according to location of the respective experiments), following guidelines described in the US National Institutes of Health Guide for the Care and Use of Laboratory Animals.
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