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Oasis max extraction cartridges

Manufactured by Waters Corporation
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Sourced in Germany

The Oasis MAX extraction cartridges are a line of solid-phase extraction (SPE) products designed for sample preparation in analytical workflows. These cartridges are used to efficiently extract, concentrate, and purify analytes from various liquid samples prior to instrumental analysis.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Ms grade water, by Thermo Fisher Scientific (1 mentions) Protein low bind tubes, by Eppendorf (1 mentions) Ms grade formic acid, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions)

Close spelling variants of this product

Oasis MAX 1 cc (30 mg) extraction cartridges Oasis MAX cartridge Oasis cartridges Oasis MAX

4 protocols using oasis max extraction cartridges

1

Trypsin Digestion and IGP Enrichment

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Proteins (500 μg) were reduced using 10 mM DTT at 56 °C for 45 min and alkylated with 20 mM iodoacetamide in the dark for 30 min at room temperature. The solutions were diluted 8-fold in 40 mM ammonium bicarbonate, and sequencing grade trypsin (Beijing Shengxia, China) was added to the solution at 1:40 (w/w), followed by incubation overnight at 37 °C. The peptides were desalted using a C18 Sep-Pak column. Twenty micrograms of desalted peptides were reserved for peptide detection, and the remaining peptides were subjected to the enrichment of IGPs using the Oasis MAX extraction cartridges (Waters), as previously described [18 (link)].
Wen P., Chen J., Zuo C., Gao X., Fujita M, & Yang G. (2022). Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B. Cells, 11(18), 2775.
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2

Quantification of Angiotensin II Levels

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Standard angiotensin II was purchased from Sigma-Aldrich (Hamburg, Germany). Stable isotope labeled angiotensin II (13C and 15 N labeled arginine) was synthesized by Campro Scientific GmbH (Berlin, Germany). MS-grade water and acetonitrile were purchased from Thermo Fisher Scientific (Ulm, Germany). MS-grade formic acid, CN-Br activated sepharose resin beads, protease inhibitor cocktail and PBS were purchased from Sigma-Aldrich (Hamburg, Germany). Monoclonal anti-angiotensin II-antibodies were purchased from Bertin Pharma (Montigny le Bretonneux, France). Low protein-bind tubes were obtained from Eppendorf (Hamburg, Germany). The Oasis MAX extraction cartridges were purchased from Waters (Eschborn, Germany).
Schulz A., Jankowski J., Zidek W, & Jankowski V. (2014). Absolute quantification of endogenous angiotensin II levels in human plasma using ESI-LC-MS/MS. Clinical Proteomics, 11(1), 37.
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3

N-Linked Glycopeptide Enrichment

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Oasis MAX extraction cartridges (Waters) were applied for enrichment of N-linked glycopeptides. Cartridges were sequentially conditioned in ACN three times, 100 mM triethylammonium acetate three times, water five times and finally 95% ACN (v/v) 1% TFA (v/v) five times. Samples were loaded twice. The non-glycopeptides were washed off by 95% ACN (v/v) 1% TFA (v/v) three times. Finally, glycopeptides were eluted in 50% ACN (v/v) 0.1% TFA (v/v), dried in a speed-vac, and desalted by C18 solid-phase extraction.
Yang G., Hu Y., Sun S., Ouyang C., Yang W., Wang Q., Betenbaugh M, & Zhang H. (2018). Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells. Analytical chemistry, 90(24), 14294-14302.
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4

Glycopeptide Enrichment and Fractionation

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The glycopeptides were enriched by mixed anion exchange (MAX) extraction cartridges and then fractionated by basic reversed-phase liquid chromatography (bRPLC) (Yang et al., 2018 (link)). In brief, 1 mg peptides were loaded into one Oasis MAX extraction cartridges (Waters) and about 200 µg glycopeptide eluate was collected together from three MAX cartridges. Then, the collected glycopeptides were desalted by C18 solid-phase extraction and injected into Zorbax Extend-C18 analytical column containing 1.8 μm particles at a flow rate of 0.2 ml/min. The glycopeptides were fractionated and collected into 96-well plates in a time-based mode from 16 to 112 min. The resulting 96 fractions of tryptic glycopeptide samples were further concentrated into 24 fractions by combining fractions 1, 25, 49, 73; 2, 26, 50, 74; etc. The 24 fraction samples were then dried in a speed-vac and stored at −80°C until LC-MS/MS analysis.
Yang G., Wang Q., Chen L., Betenbaugh M.J, & Zhang H. (2021). Glycoproteomic Characterization of FUT8 Knock-Out CHO Cells Reveals Roles of FUT8 in the Glycosylation. Frontiers in Chemistry, 9, 755238.
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