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Glun2b antibody

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The GluN2B antibody is a laboratory reagent used to detect and study the GluN2B subunit of the NMDA receptor in biological samples. It is a specific and sensitive tool for researchers investigating the expression, localization, and function of this important neuronal receptor.

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Lab products found in correlation

Protein g magnetic dynabeads, by Thermo Fisher Scientific (2 mentions)

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GluN2B (19 citations)

2 protocols using glun2b antibody

1

Immunoprecipitation of Klotho Protein

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Immunoprecipitation (IP) of klotho was performed using modification of methods described previously (Bonifacino et al., 2001 ). In brief, hippocampal lysates were incubated with magnetic Dynabeads protein G (Thermo Fisher Scientific) conjugated with GluN2B antibody (2 μg, 06600, Millipore) For specificity of bands identified by IP, GluN2B antibody (Ab) was incubated with a blocking peptide (KFNGSSNGHVYEKLSSIESDV, 21 aa, generated by Watsonbio Sciences) harboring the GluN2B antibody epitope sequence prior to conjugation with Dynabeads. Beads were magnetically pulled, washed, and eluted. The precipitates were resolved on SDS-PAGE gel. See the Supplemental Experimental Procedures for further details.
Leon J., Moreno A.J., Garay B.I., Chalkley R.J., Burlingame A.L., Wang D, & Dubal D.B. (2017). Peripheral Elevation of a Klotho Fragment Enhances Brain Function and Resilience in Young, Aging, and α-Synuclein Transgenic Mice. Cell reports, 20(6), 1360-1371.
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2

Immunoprecipitation of Klotho Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoprecipitation (IP) of klotho was performed using modification of methods described previously (Bonifacino et al., 2001 ). In brief, hippocampal lysates were incubated with magnetic Dynabeads protein G (Thermo Fisher Scientific) conjugated with GluN2B antibody (2 μg, 06600, Millipore) For specificity of bands identified by IP, GluN2B antibody (Ab) was incubated with a blocking peptide (KFNGSSNGHVYEKLSSIESDV, 21 aa, generated by Watsonbio Sciences) harboring the GluN2B antibody epitope sequence prior to conjugation with Dynabeads. Beads were magnetically pulled, washed, and eluted. The precipitates were resolved on SDS-PAGE gel. See the Supplemental Experimental Procedures for further details.
Leon J., Moreno A.J., Garay B.I., Chalkley R.J., Burlingame A.L., Wang D, & Dubal D.B. (2017). Peripheral Elevation of a Klotho Fragment Enhances Brain Function and Resilience in Young, Aging, and α-Synuclein Transgenic Mice. Cell reports, 20(6), 1360-1371.
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