Anti cxcr4 antibody

Manufactured by R&D Systems

The Anti-CXCR4 antibody is a laboratory reagent used to detect and analyze the expression of the CXCR4 chemokine receptor. CXCR4 is a G protein-coupled receptor that plays a role in various cellular processes. The antibody can be used in techniques such as flow cytometry, immunohistochemistry, and Western blotting to study CXCR4 expression in different cell types and biological samples.

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Lab products found in correlation

Cxcl12, by Thermo Fisher Scientific (1 mentions) Horse serum, by Vector Laboratories (1 mentions) Avidin biotin blocking kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CXCR4 (29 citations) Anti-CXCR4 (6 citations) CXCR4 antibody (2 citations) Anti-human CXCR4 antibody (2 citations) Mouse monoclonal anti-human CXCR4 antibody (12G5; (2 citations) Anti-human CXCR4 monoclonal antibody (2 citations)

2 protocols using anti cxcr4 antibody

PBMC Migration Assay with CXCL12

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Migration assay was performed in triplicates using 5-μm pore size Transwells (Costar). The lower compartment was filled with 600 μl of conditioned medium produced by MM cells cultured in the absence or presence of BMSCs, or 1% FCS RPMI 1640 medium containing CXCL12 (50-500 ng/ml) (PeproTech EC), and 5×10⁵PBMCs in 100 μl of 1% FCS RPMI1640 medium were applied to the upper compartment. The numberof cells migrating within 4 hours to the lower compartment was determined by FACS using CD3 and CD14 differential staining and expressed as a percentage of the input. For CXCR4 neutralization, PBMCs were pre-treated with anti-CXCR4 antibody (10 μg/ml) (R&D Systems) for 30 minutes prior to migration.

Beider K., Bitner H., Leiba M., Gutwein O., Koren-Michowitz M., Ostrovsky O., Abraham M., Wald H., Galun E., Peled A, & Nagler A. (2014). Multiple myeloma cells recruit tumor-supportive macrophages through the CXCR4/CXCL12 axis and promote their polarization toward the M2 phenotype. Oncotarget, 5(22), 11283-11296.

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Immunohistochemical Detection of CXCR4

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The avidin-biotin complex method was used to detect the proteins CXCR4 with anti-CXCR4 antibody (R&D System) at dilution 1:500. Formalin-fixed and paraffin-embedded tissues were deparaffinized and subsequently microwaved in 10 mM of sodium citrate buffer (pH 6.0) to retrieve antigens. After pre-incubation with hydrogen peroxide, avidin/biotin blocking kit (Invitrogen Co, CA), and horse serum (Vector Laboratories), the primary antibodies were applied overnight at 4 °C temperature. After incubation with the secondary antibody (rabbit anti-mouse biotinylated; Invitrogen Co, CA), the avidin-biotin complex was added and the enzyme activity was visualized with diaminobenzidine (ABC kit, Vector laboratories). Counterstaining was performed with hematoxylin.

Oum Y.H., Kell S.A., Yoon Y., Liang Z., Burger P, & Shim H. (2020). Discovery of novel aminopiperidinyl amide CXCR4 modulators through virtual screening and rational drug design. European journal of medicinal chemistry, 201, 112479.

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