Co-IP assays were performed by transfecting HEK-293T cells with the indicated plasmids using lipofectamine™ 2000 (Invitrogen). After 48 hours, cells were harvested. Cell extracts were subjected to immunoprecipitation with either the anti-Myc antibody (1/2000, Roche, 11667149001), anti-His (1/2000, GenScript, A00174) or anti-Flag (1/2000, Agilent Technologies, 200472) antibody as indicated for overnight at 4°C. The antibody was coupled to protein A/G PLUS-Agrose (Santa Cruze, sc-2003). The immunoprecipitates were washed eight times with washing buffer and analyzed by SDS-PAGE and immunoblotting with antibody as indicated. For in vivo analysis, Nuclear extracts from E13.5 limb were immunoprecipitated with anti-Foxp1 antibody (Millipore, ABE68), anti-Foxp2 antibody (Abcam, ab16046) or IgG (Santa Cruze, sc-2027), and then blotted with anti-Foxp1 (1/1000, Millipore, ABE68), anti-Foxp2 (1/2000, Abcam, ab16046), anti-Foxp4 antibody (1/1000, Milipore, ABE74) and anti-Runx2 antibody (1/1000, Santa Cruze, sc-10758) as indicated.
Ab16046
Manufactured by Santa Cruz Biotechnology
Ab16046 is a laboratory reagent product manufactured by Santa Cruz Biotechnology. It is a polyclonal antibody intended for use in various research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab16046, by Merck Group (1 mentions)
Protein a g plus agrose, by Santa Cruz Biotechnology (1 mentions)
Abe68, by Abcam (1 mentions)
Anti myc antibody, by Roche (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Ab54583, by Abcam (1 mentions)
Ab72418, by Abcam (1 mentions)
Vectastain abc reagent, by Vector Laboratories (1 mentions)
Bx41 microscope, by Olympus (1 mentions)
2 protocols using ab16046
1
Co-Immunoprecipitation Assay Protocol
2
Immunohistochemical Analysis of Mouse Lung Development
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Lungs from wild type mouse embryos from E11 till E18 were dissected and fixed in 4% PFA overnight at 4°C before processing for paraffin embedding. Sections of 5 μm were dewaxed and rehydrated, followed by antigen retrieval with microwave treatment in 10 mM Tris-HCl, 1 mM EDTA pH9.0 (TE). After quenching endogenous peroxidase with 1.5% H2O2, samples were blocked with 1% BSA, 0.05% Tween20 in PBS for 10 min at room temperature and then incubated with antibody against CHD4 (Abcam; ab72418), FOXP2 (Abcam; ab16046), FOXP4 (Santa Cruz; sc-292474), or CUX1 (Abcam, Ab54583) diluted in blocking buffer at 4°C overnight. After washing in PBS with 0.05% Tween20, slides were incubated in blocking buffer with biotin conjugated secondary antibody for 30 min at room temperature. Then samples were incubated with VECTASTAIN® ABC Reagent (Vector) for 30 min, followed by incubation with diaminobenzidine (Fluka). Slides were counterstained with hematoxylin and mounted with pertex. Sections were analyzed using Olympus BX41 microscope.
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