Log In Sign up
The largest database of trusted experimental protocols

Caspase 7

Manufactured by Merck Group
Visit Supplier

Caspase 7 is a lab equipment product manufactured by Merck Group. It is an enzyme that plays a crucial role in the process of apoptosis, a form of programmed cell death. Caspase 7 is responsible for the cleavage of various cellular proteins, which leads to the dismantling of the cell and its eventual death.

Automatically generated - may contain errors

Lab products found in correlation

X tremegene 9, by Roche (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Mouse anti ezrin, by Thermo Fisher Scientific (2 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (2 mentions) Rabbit anti caspase 7, by Cell Signaling Technology (2 mentions) Rabbit anti annexin a2, by BD (2 mentions) Rabbit anti listeria, by BD (2 mentions) Rabbit anti lm antibody, by Abcam (2 mentions) Mouse anti phosphatidylserine, by Abcam (2 mentions) Annexin 5 alexa fluor 488 and 647 conjugates, by Thermo Fisher Scientific (2 mentions) Fluorescent secondary antibodies alexafluor conjugates, by Thermo Fisher Scientific (2 mentions) Annexin a1, by Merck Group (2 mentions) Annexin a2, by Merck Group (2 mentions) Annexin a6, by Merck Group (2 mentions) Caspase 9, by Merck Group (1 mentions) Cleaved parp, by Merck Group (1 mentions) Caspase 3, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Muse Caspase-3/7 Kit (36 citations) Muse Caspase-3/7 Assay Kit (27 citations) Caspase-3/7 assay kit (4 citations) Caspase 3/7 reagent (4 citations) CaspaTag Caspase 3/7 in situ Assay Kit (4 citations) Caspase 7-AAD (3 citations) Muse® Caspase 3/7 Activation Kit (2 citations) Caspase-3/7 Kit (2 citations) Anti-caspase 7 (2 citations)

3 protocols using caspase 7

1

Western Blot Analysis of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell pellets were lysed in RIPA buffer (150 nM NaCl, 1% (v/v) IGEPAL, 0.5% (w/v) deoxycholic acid, 0.1% (w/v) SDS, 50 mM Tris) supplemented with protease and phosphatase inhibitors. Protein was quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Lysates were electrophoresed on acrylamide gels of appropriate acrylamide percentage, transferred onto PVDF membranes and immunoblotted using the following antibodies: LIMD1 (in house), β-actin (Sigma #A1978), Total PARP (CST #9532), Cleaved PARP (CST #6704), Caspase 7 (CST #12827), Caspase 9 (CST #9508), Caspase 3 (CST #9668) and Cleaved Caspase 9 (CST #7237), Chk1 (CST #2360). Anti-IgG horseradish peroxidase (Dako) and chemiluminescent detection (Thermo Fisher Scientific) were used to develop immunoblots.
Davidson K., Grevitt P., Contreras-Gerenas M.F., Bridge K.S., Hermida M., Shah K.M., Mardakheh F.K., Stubbs M., Burke R., Casado P., Cutillas P.R., Martin S.A, & Sharp T.V. (2021). Targeted therapy for LIMD1-deficient non-small cell lung cancer subtypes. Cell Death & Disease, 12(11), 1075.
+ Open protocol
+ Expand
2

Fluorescence Microscopy Protocols for Annexin and Listeria Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used were rabbit anti-Annexin A2 (#610068 from BD Biosciences), rabbit anti-GFP (#A11120 from Invitrogen), rabbit anti-Caspase 7 (#12827 from Cell Signaling), rabbit anti-Listeria (#B223021 from BD Biosciences), rabbit anti-Lm antibody (from Dr. Pascale Cossart, Institut Pasteur, France), mouse anti-phosphatidylserine (#18005 from Abcam) and mouse anti-ezrin (#35-7300 from Invitrogen). Rat anti-TIM4 blocking antibody was previously described37 . Alexa Fluor 568 Phalloidin, Annexin V-Alexa Fluor-488 and -647 conjugates and all fluorescent secondary antibodies (AlexaFluor conjugates) were from Invitrogen. DAPI (#D1306 from Invitrogen) was used at 1:2500 dilution to stain the nuclei where indicated. Cytochalasin D (#2502555; 10 μM final) and Latrunculin B (#428020; 10μg/mL final) were from Calbiochem. For transfection of HeLa cells, Xtreme Gene 9 (Roche) transfection reagent was used as per manufacturer’s protocols. LifeAct-mRFP38 was a gift from Dr. Ray Truant (McMaster University, Canada). The following siRNAs were from Sigma: Annexin A1 (#00157996), Annexin A2 (#00246294), Annexin A6 (#00063383), Caspase 7 (#00128361).
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
+ Open protocol
+ Expand
3

Fluorescence Microscopy Protocols for Annexin and Listeria Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used were rabbit anti-Annexin A2 (#610068 from BD Biosciences), rabbit anti-GFP (#A11120 from Invitrogen), rabbit anti-Caspase 7 (#12827 from Cell Signaling), rabbit anti-Listeria (#B223021 from BD Biosciences), rabbit anti-Lm antibody (from Dr. Pascale Cossart, Institut Pasteur, France), mouse anti-phosphatidylserine (#18005 from Abcam) and mouse anti-ezrin (#35-7300 from Invitrogen). Rat anti-TIM4 blocking antibody was previously described37 . Alexa Fluor 568 Phalloidin, Annexin V-Alexa Fluor-488 and -647 conjugates and all fluorescent secondary antibodies (AlexaFluor conjugates) were from Invitrogen. DAPI (#D1306 from Invitrogen) was used at 1:2500 dilution to stain the nuclei where indicated. Cytochalasin D (#2502555; 10 μM final) and Latrunculin B (#428020; 10μg/mL final) were from Calbiochem. For transfection of HeLa cells, Xtreme Gene 9 (Roche) transfection reagent was used as per manufacturer’s protocols. LifeAct-mRFP38 was a gift from Dr. Ray Truant (McMaster University, Canada). The following siRNAs were from Sigma: Annexin A1 (#00157996), Annexin A2 (#00246294), Annexin A6 (#00063383), Caspase 7 (#00128361).
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!