The human EC cell lines RL95-2 and HEC-1A were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were cultured in Dulbecco modified Eagle medium/F12 (Thermo Scientific, Rockford, IL, USA) and McCoy 5A medium (Thermo Scientific), respectively with 10% fetal bovine serum (FBS) (Thermo Scientific). The KLE cell line was obtained from the China Center for Type Culture Collection (Wuhan, China) and was cultured in Dulbecco modified Eagle medium/F12 with 10% FBS. All cells were incubated at 37 °C in a humidified incubator with 5% CO2.
Dulbecco modified eagle medium f12
Manufactured by Thermo Fisher Scientific
Sourced in United States
Dulbecco Modified Eagle Medium/F12 is a cell culture medium used to support the growth and maintenance of a variety of cell types. It is a balanced salt solution that provides essential nutrients, vitamins, and other components required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Kle cell line, by National Collection of Authenticated Cell Cultures (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Lonsera (2 mentions)
Mccoy 5a medium, by Thermo Fisher Scientific (1 mentions)
Hec 1a, by American Type Culture Collection (1 mentions)
Rl95 2, by American Type Culture Collection (1 mentions)
Rl95 2 cell line, by American Type Culture Collection (1 mentions)
Liberate blendzyme 2, by Roche (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Ng108 15, by American Type Culture Collection (1 mentions)
Neural basal media a, by Thermo Fisher Scientific (1 mentions)
Laminin coated glass coverslips, by Thermo Fisher Scientific (1 mentions)
Leibovitz s l 15 media, by Thermo Fisher Scientific (1 mentions)
Ca2 mg2 free hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
Endothelium cell medium, by ScienCell (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Enzyme linked immunosorbent assay kit, by USCN (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
7180 automatic analyzer, by Hitachi (1 mentions)
13 protocols using dulbecco modified eagle medium f12
1
Cell Culture Protocols for Human EC Lines
2
Culturing Human Endometrial Cancer Cells
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The RL95-2 cell line was obtained from American Type Culture Collection (ATCC). The KLE cell line was obtained from China Center for Type Culture Collection. The human endometrial cancer cell lines RL95-2 and KLE cells were cultured in Dulbecco modified Eagle medium/F12 (Thermo Fisher Scientific) containing 10% (v/v) heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific) at 37 °C in a humidified atmosphere containing 5% CO2.
3
Culturing Diverse Neuronal Cell Lines for Electrophysiology
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Human embryonic kidney 293 (HEK293) cell lines stably expressing human wide-type CaV3.2 (HEK3.2), CaV3.1 (HEK3.1), and CaV3.3 (HEK3.3) (Kerafast, Boston, MA), CaV2.2 (HEK2.2, provided by Dr. Missler at Georg-August University, Germany),21 (link) and neuronal NG108-15 (ATCC, Manassas, VA) were cultured in Dulbecco modified Eagle medium supplemented with glutaMAX, 10% fetal bovine serum (ThermoFisher, Rockford, IL), and antibiotics using standard techniques. Dissociated DRG neuronal culture for electrophysiology was performed as previously described.59 (link) In brief, DRG (L4 and L5) from male rats were rapidly harvested from the isoflurane-anesthetized animals and were incubated in 0.01% liberate blendzyme 2 (Roche Diagnostics, Madison, WI) for 30 minutes, followed by incubation in 0.25% trypsin and 0.125% DNase for 30 minutes, both dissolved in Dulbecco modified Eagle medium/F12 with glutaMAX (ThermoFisher). After exposure to 0.1% trypsin inhibitor and centrifugation, the pellet was gently triturated in culture medium containing Neural basal media A (ThermoFisher) and 0.5 μm glutamine. Dissociated cells were plated onto 5% laminin-coated glass coverslips (ThermoFisher), maintained at 37°C in humidified 95% air and 5% CO2, and were studied in approximately 6 to 8 hours after harvest in electrophysiological experiments.
4
Dissociation and Culture of Murine Trigeminal Ganglia
5
FAdV Isolation, Propagation, and Antibody Generation
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The FAdV-4 strain SD and the FAdV-8b strain JSSQ15 were isolated and stored in our laboratory and propagated in leghorn male hepatoma (LMH) cells [7 (link)]. The FAdV-8a strain AH720 was kindly provided by Professor Hongjun Chen. The recombinant virus FA4-EGFP was generated by our laboratory [20 (link)]. LMH cells from the American strain collection center (ATCC) were cultured in Dulbecco Modified Eagle Medium/F12 (Gibco, NY, USA) containing 10% fetal bovine serum, and placed in a 5% CO2 incubator at 37 °C. Monoclonal antibody (mAb) 5F10 against fiber of FAdV-8b, mAb 3B5 against the Fiber-1 of FAdV-4, and chicken sera against FAdV-4 were generated and stored in our laboratory. mAb 1B5 against the hexon of FAdVs, mAb 1C9 against Fiber-2 of FAdV-4, and chicken sera against FAdV-8a strain AH720 were kindly provided by Professor Hongjun Chen. The pMD19- HAL-EGFP-F2-HAR simple vector was constructed and stored in our laboratory [20 (link)].
6
Cervical Cancer Organoid Development
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SiHa, HeLa, and HUVEC cell lines were obtained from American Type Culture Collection. SiHa and HeLa cells were grown in minimum essential medium and RPMI‐1640 supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific), 100 U mL−1 of penicillin, 100 mg mL−1 of streptomycin, and 250 ng of amphotericin B (NCM Biotech). HUVECs were cultured in an endothelial cell growth medium (Endothelium Cell Medium, ScienCell) supplemented with 5% FBS (Gibco). Primary CAFs were isolated as described previously[46 ] and cultured in the Dulbecco Modified Eagle Medium/F12 (Gibco) supplemented with 10% FBS. All cells were cultured at 37 °C in an atmosphere of 5% carbon dioxide. CS Well 600 chambers (JIYAN, China), which contained 261 wells per chamber, were used to fabricate the 3D organoids. A total of 1000 SiHa/HeLa cells, HUVECs and CAFs per well were mixed in a proportion of approximately 9:3:1, seeded into the chambers and cultured for 48 h to generate organoids.
7
Cell Culture and Viral Strains Analysis
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Leghorn Male Hepatoma cell line (LMH) from the American strain collection center (ATCC) was cultured in Dulbecco Modified Eagle Medium/F12 (Gibco, NY, USA) containing 10% fetal bovine serum (Lonsera, Shanghai, China) at 37°C with 5% CO2. The DAdV-3 strain GD and the FAdV-4 strain SD were isolated and stored in our laboratory. Monoclonal antibody (mAb) 3D9 and 5C3 against Fiber-2 of DAdV-3 were prepared and stored in our laboratory. mAb 1B5 against Hexon of FAdV-4 and mAb 1C9 against Fiber-2 of FAdV-4 were prepared and stored in our laboratory. The pMD19-HAL-LoxP-RFP-LoxP-HAR simple vector carrying the RFP expression cassette was constructed and stored in our laboratory. The vector pcDNA3.1-Cre was constructed and stored in our laboratory.
8
Isolation and Propagation of FAdV-4 in LMH Cells
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The FAdV-4 strain SD was isolated and stored in our laboratory and propagated in leghorn male hepatoma (LMH) cells. LMH cells were purchased from ATCC and cultured in Dulbecco Modified Eagle Medium/F12 (Gibco, NY, USA) supplemented with 10% fetal bovine serum (Lonsera, Shanghai, China) in a 5% CO2 incubator at 37 ℃. Monoclonal antibody (mAb) 3B5 against Fiber-1, and mAb 3C2 against Fiber-2 were generated in our laboratory, and mAb 3C2 against Fiber-2 were generated in our laboratory, and Professor Hongjun Chen kindly provided mAb 1C9 against Fiber-2.
9
Culture of Patient-Derived Cells
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Patient-derived cells were cultured in Dulbecco modified Eagle medium/F-12 (GIBCO BRL, USA) supplemented with heat-inactivated fetal bovine serum (20% [vol/vol] for myoblasts and 10% for fibroblasts; GIBCO BRL).
10
Primary Rat ONH Astrocyte Culture
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The primary culture rats ONH astrocytes were purchased from Procell Life Science & Technology Co (Wuhan, China). Then the cells were cultured at 37 °C and 5% CO2 in Dulbecco Modified Eagle Medium F/12 (GIBCO, United States) supplemented with 10% fetal bovine serum (FBS, GIBCO, United States) and 1% penicillin/ streptomycin (Hyclone, United States). The cells were grown to confluence and split in this media until the fourth generation to obtain enough viable cells for the experiment. A third cell line was grown to be used for validation purposes using immunohistochemistry.
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