Poly 3 hydroxybutyrate phb

Poly(3-hydroxybutyrate) (PHB) was supplied by Sigma-Aldrich Chemical Co. Prior to PHB film formation in a thermomechanical hydraulic press, PHB underwent a heat treatment in a miniextruder/compounder (HAAKE MiniLab) similar to that used, when PHB nanocomposites are prepared. The extruded PHB pieces were heated in a vacuum drying oven at 175°C for 3 min and then they were pressed in the hydraulic press under 200 atm for 3 minutes at 175°C.
All the other chemicals were supplied by Sigma-Aldrich Chemical Co. and were used as they were received.

Poly-3-hydroxybutyrate (PHB), gelatin (bovine skin, type B) (Gel), glutaraldehyde (GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St Louis, MO, USA). 2,2,2-trifluoroethanol(TFE) and metronidazole (MTZ) were purchased from Acros-organics (Thermo Fisher Scientific, Waltham, MA, USA). The L-929 cell line was purchased from the Typical Culture Collection Committee Cell Bank, Chinese Academy of Sciences (Beijing, China). Cell counting kit-8(CCK-8)(2-(2-methoxy-4-nitrophenyl)-3-(4-Nitphenyl)-5-(2,4-disulfophenyl)-2H-tetrazole-monosodium salt) was procured from Beijing Solar bio-Science & Technology Co., Ltd., Beijing, China. Fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (PBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco-BRL (Grand Island, NE, USA). All the materials and reagents were analytical grade and were used as received without further purification.