Anti cd34 antibody sc 74499

Immunohistochemical localization of α-smooth muscle actin (α-sma), CD34, vascular endothelial grow factor (VEGF) and Ki67 was performed in endometriosis lesions as already described [52 (link)]. The sections were incubated overnight with primary antibodies: anti-α-sma antibody (CGA7, Santa Cruz Biotechnology, Heidelberg, Germany), anti-CD34 antibody (sc-74499, Santa Cruz Biotechnology, Heidelberg, Germany), anti-VEGF antibody (sc-7269, Santa Cruz Biotechnology, Heidelberg, Germany) and anti-Ki67 antibody (sc-23900, Santa Cruz Biotechnology, Heidelberg, Germany). All sections were washed with PBS and then treated as previously reported [53 (link)]. Stained sections were observed using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) following a typical procedure [54 (link)]. The histogram profile is related to the positive pixel intensity value obtained [55 (link)].

Anti-NOX4 antibody (ab133303), anti-Iba-1 antibody (ab15690), anti-8-Oxo-2′-deoxyguanosine (anti-8-OHdG) antibody (ab48508), anti-3-Nitrotyrosine (3-NT) antibody (ab61392), anti-Bcl-2 antibody (ab59348) were purchased from Abcam (Cambridge, MA, United States). Anti-NEUN antibody (#MAB377) and anti-glial fibrillary acidic protein (anti-GFAP) antibody (#MAB360) were purchased from Merck Millipore (Bedford, MA, United States). Anti-Bax antibody (#14796) and anti-caspase-3 antibody (#14220) were purchased from Cell Signaling Technology (Danvers, MA, United States). Anti-ZO-1 antibody (21773-1-AP), anti-occludin antibody (27260-1-AP), anti-matrix metalloproteinase-9 (anti-MMP-9) antibody (10375-2-AP), and anti-NEUN antibody (26975-1-AP) were purchased from Proteintech Biotechnology (Rosemont, IL, United States). Anti-claudin-5 antibody (#AB40754) was purchased from Absci Biotechnology (Vancouver, WA, United States). Anti-CD34 antibody (sc-74499) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States).