Mhcii alexa 700

Manufactured by Thermo Fisher Scientific

MHCII-Alexa-700 is a fluorescent-labeled antibody that specifically binds to major histocompatibility complex class II (MHCII) proteins. It is designed for use in flow cytometry and other immunoassay applications to detect and analyze MHCII-expressing cells.

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Lab products found in correlation

Rbc lysis, by Merck Group (1 mentions) Rat anti mouse cd68, by Bio-Rad (1 mentions) Cd44 fitc, by BD (1 mentions) Cd86 fitc, by BD (1 mentions) F4 80 pe, by BioLegend (1 mentions) Cd19 bv605, by BioLegend (1 mentions) Cd11c pe cy7, by BioLegend (1 mentions) Cd8 alexa700, by BD (1 mentions) Cd4 apc efluor 780, by Thermo Fisher Scientific (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Ly6g apc cy7, by BioLegend (1 mentions) Cd5 pe cy7, by BioLegend (1 mentions) Ly6c percp cy5, by BD (1 mentions) Cd62l pe cy5, by BioLegend (1 mentions) Cd11b efluor450, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MHCII (84 citations) Alexa Fluor 700-anti-MHCII (3 citations) MHCII-Alexa Fluor 700 (2 citations)

2 protocols using mhcii alexa 700

Flow Cytometric Immune Cell Profiling

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Single cell suspensions from harvested tissues were prepared for flow cytometry as previously described (23 (link)). After red blood cell (RBC) lysis (Sigma), single-cell suspensions were incubated for 30 minutes on ice with the following antibodies: CD45-APC, CD11b-APC or CD11b-e450, Gr-1-PerCPCy5.5, Ly6C-FITC, F4/80-PE-Cy7 or F4/80-e450, MHCII-Alexa-700, and CD115 (CSF1R)-PE conjugated antibodies, 1:200 (eBioscience), followed by two washes with 2% FBS in PBS (FACS buffer). Cells were fixed in 3% PFA for 15 min at room temperature and washed two times with FACS buffer. Cell acquisition was done on a BD LSR-II flow cytometer (Beckman Coulter). Data were analyzed with FlowJo software (TreeStar) (23 (link)).

Escamilla J., Schokrpur S., Liu C., Priceman S.J., Moughon D., Jiang Z., Pouliot F., Magyar C., Sung J.L., Xu J., Deng G., West B.L., Bollag G., Fradet Y., Lacombe L., Jung M.E., Huang J, & Wu L. (2015). CSF1 Receptor Targeting In Prostate Cancer Reverses Macrophage-Mediated Resistance To Androgen Blockade Therapy. Cancer research, 75(6), 950-962.

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Multicolor Immunofluorescence and Flow Cytometry

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Antibodies used for immunofluorescence were rat anti-mouse CD68 (Serotec), hamster anti-mouse CD11c (N418, Biolegend), rabbit polyclonal anti-Brucella LPS (gift from Edgardo Moreno, Universidad Nacional, Costa Rica).
Antibodies for flow cytometry were MHCII-Alexa700 (eBioscience), CD11b-eFluor 450 (eBioscience), Ly6C-PerCP-Cy5.5 (BD Bioscience), Ly6G-APC-Cy7 (Biolegend), CD11c-PE-Cy7 (Biolegend), F4/80-PE (Biolegend), CD86-FITC, CD8-Alexa700 (BD Bioscience), CD4-APC-eFluor780 (eBioscience), CD19-BV-605 (Biolegend), CD62L-PE-Cy5 (Biolegend), CD5-PE-Cy7 (Biolegend), and CD44-FITC (BD Bioscience). Live cells were distinguished by staining with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life technologies).

von Bargen K., Gagnaire A., Arce-Gorvel V., de Bovis B., Baudimont F., Chasson L., Bosilkovski M., Papadopoulos A., Martirosyan A., Henri S., Mège J.L., Malissen B, & Gorvel J.P. (2015). Cervical Lymph Nodes as a Selective Niche for Brucella during Oral Infections. PLoS ONE, 10(4), e0121790.

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