Anti sesn2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Sesn2 is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that specifically recognizes and binds to the Sesn2 protein. Sesn2 is a key regulator involved in various cellular processes. The Anti-Sesn2 antibody can be used for the detection and analysis of Sesn2 expression in biological samples through techniques such as western blotting, immunohistochemistry, and immunoprecipitation.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

SESN2 (2 citations)

2 protocols using anti sesn2

Western Blot Analysis of Senescence and ECM Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The protein concentration in the supernatant was determined by BCA assay. Aliquots of 80 μg protein were separated in SDS-PAGE and transferred onto nitrocellulose membranes by an electric transfer (BIORAD Inc., USA). The membranes were blocked with 5% milk PBS-Tween20 (PBST) and incubated with primary antibody including: anti-Sesn1 (Abcam, USA), anti-Sesn2 (Santa Cruz Biotechnology, USA), anti-Sesn3 (Abcam, USA), anti-COL I (Abcam, USA), anti-COL III (Abcam, USA), anti-FN1 (Abcam, USA), and anti-Actin (Cell Signaling, USA) antibodies. They were then incubated with Alexa Fluor 700 antibodies (Molecular Probes, USA). Images were captured on an Odyssey Infrared Imaging System (LI-COR Biosciences, USA), and quantified using Odyssey v1.2.

Dong Z., Lin C., Liu Y., Jin H., Wu H., Li Z., Sun L., Zhang L., Hu X., Wei Y., Wang C, & Han W. (2017). Upregulation of sestrins protect atriums against oxidative damage and fibrosis in human and experimental atrial fibrillation. Scientific Reports, 7, 46307.

+ Open protocol

+ Expand

Antioxidant and Apoptosis Regulation in NPC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two human NPC cell lines, CNE1 and CNE2, were obtained from the Molecular Medicine and Cancer Research Center, Chongqing Medical University. All cells were grown in RMPI-1640 medium (HyClone, Logan City, UT, USA) with 10% fetal bovine serum (HyClone) at 37°C with 5% CO₂. NAC was purchased from Beyotime (Beyotime Institute of Biotechnology, Nantong, P.R. China), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), ADR, cisplatin, and 5-FU were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine RNAiMAX was purchased from Invitrogen (Carlsbad, CA, USA), and human p53 siRNA was purchased from GenePharma (Shanghai, P.R. China). The antibodies used in this study were anti-NFBD1 (Abcam, UK); anti-PARP, anti-cleaved caspase 3, anti-caspase 9, anti-Bcl-2, and anti-Bcl-xl (Cell Signaling Technology, Danvers, MA, USA); anti-cytochrome c, anti-SESN2, anti-GPX-1/2, and ALDH4A1 (Santa Cruz Biotechnology, USA); and anti-PUMA, anti-P53, anti-MDM2, anti-Bax, anti-SOD2, and anti-PA26 (Bioworld Technology, USA).

Wang Z., Liao K., Zuo W., Liu X., Qiu Z., Gong Z., Liu C., Zeng Q., Qian Y., Jiang L., Bu Y., Hong S, & Hu G. (2017). Depletion of NFBD1/MDC1 Induces Apoptosis in Nasopharyngeal Carcinoma Cells Through the p53–ROS–Mitochondrial Pathway. Oncology Research, 25(1), 123-136.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!