Laser doppler flowmetry probe

The endovascular perforation was produced as previously described with modifications8 (link). Anesthesia was induced with sodium pentobarbital (80 mg/kg). After anesthesia of intraperitoneal injection in mice, a laser Doppler flowmetry probe (Moor Instruments Inc., Wilmington, DE) was adhered at the left temporal bone to monitor the blood flow of the left hemispherea. The left common carotid artery (CCA), internal carotid artery (ICA) and external carotid artery (ECA) were exposed and the external carotid artery (ECA) was fashioned into a 3-mm stump. A 6-0 monofilament nylon suture was advanced into the internal carotid artery (ICA) from the ECA stump until resistance was felt, about 1.0 cm from Common carotid artery bifurcation have resistance to insert again after 2 mm pricking blood vessels and then the suture was immediately withdrawn to induce subarachnoid hemorrhage. The cerebral blood flow decreased obviously on doppler blood flow meter monitoring. In the sham group, the procedure is similar, but not pricking blood vessels, blood flow is also no obvious change. We recorded the blood flow changes before and after the process of blood vessel puncture in all animals. The animals were returned to their cages after operation and had free access to food and water.

After evaluating the neurological deficit in the second rat experiment, CBF during cerebral ischemia was measured with a laser Doppler flowmetry probe (Moor Instruments Ltd). In brief, under anesthesia, a small incision was made in the center of the skull. Remove the underlying fascia and press it until there was no bleeding point on the skull. A small area of the skull was exposed to allow placement of the laser-Doppler probe.
The calculation formula is as follows: flux (%) = flux_ischemic/flux_{contralateral}∗100%.