To detect the expression of a variety of molecules on LP mononuclear cells, they were incubated with antibodies for 20 min. To detect the intracellular expression, after their surface molecules were stained, cells were fixed using a Cytofix/Cytoperm Kit (BD Pharmingen) and incubated with antibodies for 20 min. Multi-color flow cytometric analyses were performed using MACS (Miltenyi Biotec, Auburn, CA, USA), a Quant flow cytometer and Flowjo (FlowJo LLC, Ashland, OR, USA).
The following monoclonal antibodies (mAbs) were obtained from Biolegend (San Diego, CA, USA): APC anti-mouse CD4 (RM4-5), PE/Cy7 anti-mouse CD3 (145-2C11), PE anti-mouse CD25 (PC61), PE anti-mouse, IL-17A (TC11-18H10), FITC anti-mouse IFN-γ (XMG1.2) and Purified anti-mouse CD16/32 (93). The following mAbs were obtained from BD Pharmigen (San Diego, CA, USA): FITC anti-mouse CD45RB (16 A); PE anti-mouse Bcl-2 (3F11). The following mAbs were obtained from eBioscience (San Diego, CA, USA): FITC anti-mouse Foxp3 (FJK-16S).
The following monoclonal antibodies (mAbs) were obtained from Biolegend (San Diego, CA, USA): APC anti-mouse CD4 (RM4-5), PE/Cy7 anti-mouse CD3 (145-2C11), PE anti-mouse CD25 (PC61), PE anti-mouse, IL-17A (TC11-18H10), FITC anti-mouse IFN-γ (XMG1.2) and Purified anti-mouse CD16/32 (93). The following mAbs were obtained from BD Pharmigen (San Diego, CA, USA): FITC anti-mouse CD45RB (16 A); PE anti-mouse Bcl-2 (3F11). The following mAbs were obtained from eBioscience (San Diego, CA, USA): FITC anti-mouse Foxp3 (FJK-16S).