Monodansylcadaverine assay kit mdc

A total of 1 mL of C. gloeosporioides conidial suspension (1 × 10⁶ conidia/mL) was prepared, and FeCl₃ was added to reach a final concentration of 1/2 MIC and MIC, followed by incubation at 28 °C and 180 rpm on a shaker for 3 h before staining. Conidial viability was detected using fluorescein diacetate (FDA, 5 g L⁻¹). The samples were then incubated with the corresponding stains in the dark for 5 min, after which they were rinsed twice with PBS (pH = 7.0). The autophagosomes and intracellular ROS accumulation of the conidia were detected using a monodansylcadaverine assay kit (MDC, Solarbio, Beijing, China) and a Reactive Oxygen Species (ROS) assay kit (Solarbio, Beijing). All samples were observed under a fluorescence microscope (Nikon Eclipse 90i).

Monodansylcadaverine assay kit (MDC; Solarbio, Beijing, China) was used to detect the formation of intracellular autophagosomes. A density of 1 × 104 untreated and si-NC or si-LAMC2 transfected cells were seeded into six-well plate. After 1 day, the cells were stained with 50 μM MDC for 15 min. A fluorescence microscope was used to obtain images of the samples.