Recombinant human CXCL12β (3 nM), alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101–5) in RPMI 1640 1% FBS. As internal controls within each assay, medium or recombinant protein alone were used. MonoMac-1 cells (1.25 × 105) were placed on the upper compartment and separated from the lower chamber by a 5 μm pore size filter. The cells were incubated at 37° C for 3 h in a humidified incubator with 5% CO2. The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2, measuring absorbance at 490 nm using a Synergy H1 plate reader (Bio-Tek). The following recombinant proteins were used: SARS-CoV-2 N-His (Sino Biological # 40588-V07E), HCoV-OC43 N-His (Sino Biological # 40643-V07E), GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E), 229E N-His (Sino Biological # 40640-V07E), and HKU1 N-His (Sino Biological # 40642-V07E).
Gfp his
Manufactured by Thermo Fisher Scientific
The GFP-His is a laboratory equipment that is designed to detect and quantify the expression of proteins tagged with a 6x histidine (His) sequence. It is a versatile tool for researchers working in various fields of molecular biology and biochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (2 mentions)
Synergy h1 plate reader, by Agilent Technologies (2 mentions)
Chemotx system plate, by Neuro Probe (2 mentions)
Sars cov 2 n his, by Sino Biological (2 mentions)
Hcov oc43 n his, by Sino Biological (2 mentions)
Nl63 n his, by Sino Biological (2 mentions)
229e n his, by Sino Biological (2 mentions)
Hku1 n his, by Sino Biological (2 mentions)
2 protocols using gfp his
1
Chemotaxis assay for SARS-CoV-2 N protein
2
Chemotaxis Assay of SARS-CoV-2 Nucleocapsid Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Recombinant human CXCL12β (3 nM), alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1% FBS. As internal controls within each assay, medium or recombinant protein alone was used. MonoMac-1 cells (1.25 × 105) were placed on the upper compartment and separated from the lower chamber by a 5-µm pore size filter. The cells were incubated at 37 °C for 3 h in a humidified incubator with 5% CO2. The migrated cells in the lower chamber were stained with 5 µL CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37 °C with 5% CO2, measuring absorbance at 490 nm using a Synergy H1 plate reader (Bio-Tek). The following recombinant proteins were used: SARS-CoV-2 N-His (Sino Biological # 40588-V07E), HCoV-OC43 N-His (Sino Biological # 40643-V07E), GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E), 229E N-His (Sino Biological # 40640-V07E), and HKU1 N-His (Sino Biological # 40642-V07E).
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