Rabbit anti gapdh 2118 antibody

COS-7 cells were obtained from the American Type Culture Collection. Rabbit polyclonal antibody targeting SR-BI’s C-terminal region (NB400–101) was purchased from Novus Biologicals. Rabbit-anti-GAPDH (#2118) antibody was obtained from Cell Signaling Technology. Horseradish peroxidase (HRP)–conjugated donkey-anti-rabbit-IgG was purchased from GE Healthcare Life Sciences. Fluorescein isothiocyanate (FITC)–conjugated goat-anti-rabbit IgG secondary antibody (#554020) was from BD Biosciences. [¹²⁵I]-sodium iodide, [³H]-cholesteryl hexadecyl ether, [³H]-cholesteryl oleyl ether, and [³H]-cholesterol were purchased from PerkinElmer. Acyl-CoA cholesterol acyltransferase inhibitor (Sandoz 58–035) was obtained from MilliporeSigma. Human HDL was obtained from Alfa Aesar. Recombinant cholesteryl ester transfer protein was from Roar Biomedical. Cholesterol oxidase from Streptomyces spp. and thin-layer chromatography standards (cholesterol, 4-cholesten-3-one, and cholesteryl oleate) were obtained from Sigma-Aldrich. FuGENE 6 transfection reagent was obtained from Promega. EZ-Link Sulfo-NHS-LC-Biotin was purchased from Thermo Fisher Scientific. All other reagents were of analytical grade.

The mouse anti-ERRα (sc-65715), anti-vimentin (sc-66002), and anti-SNAI1 (sc-271977) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The mouse anti-E-cadherin antibody (M106) was purchased from Takara Bio Inc. (Shiga, Japan). The rabbit anti-SNAI1 antibody (SAB2108482) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The rabbit anti-ZEB1 antibody (GTX105278) was purchased from Gene Tex (Los Angeles, CA, USA). The rabbit anti-GAPDH (#2118) antibody and the anti-rabbit (#7074) and anti-mouse (#7076) IgG, HRP-linked antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). All antibodies were used at the concentration recommended by the manufacturers.