Mircury hy3 hy5 power labeling kit

Tissue samples. The placental tissues used in this study were obtained from women with normal pregnancies (n=20) and patients with PE (n=20) at the Department of Obstetrics and Gynecology, Tangshan Worker Hospital. All experimental protocols were approved by the Ethics Committee of the Tangshan Worker Hospital, He Bei Medical University. All studies were performed in accordance with the ethical guidelines of the Tangshan Worker Hospital, He Bei Medical University. Written informed consent was obtained from all patients. miRNA expression profiling. Total RNA was isolated from frozen placentas tissue by the miRNeasy mini kit (Qiagen, Ltd., West Sussex, UK) following the manufacturer's protocol. After passing the RNA purity and quantity measurement using the NanoDrop ND-1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURY™ Hy3™/ Hy5™ Power labeling kit and hybridized to the miRCURY LNA™ Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner. Data was analyzed using Genespring software (Agilent Technologies, Inc.). The heat map of the 54 miRNAs most obvious differences was created using a method of hierarchical clustering by GeneSpring GX, v.7.3 (Agilent Technologies, Inc.).