Dillapiole

Dimethyl sulfoxide (DMSO), fMLF, Histopaque 1077, (−)-α-thujone, α/β-thujone, and dillapiole were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Menthone, isomenthone, and pulegone were from Toronto Research Chemicals (North York, ON, Canada). n-Hexane was purchased from Merck (Darmstadt, Germany). Fluo-4AM was purchased from Invitrogen (Carlsbad, CA, USA). Hanks’ balanced salt solution (HBSS; 0.137 M NaCl, 5.4 mM KCl, 0.25 mM Na₂HPO₄, 0.44 mM KH₂PO₄, 4.2 mM NaHCO₃, 5.56 mM glucose, and 10 mM HEPES, pH 7.4) was purchased from Life Technologies (Grand Island, NY, USA). HBSS without Ca²⁺ and Mg²⁺ is designated as HBSS^–; HBSS containing 1.3 mM CaCl₂ and 1.0 mM MgSO₄ is designated as HBSS⁺.

The chemical standards used, namely ocimene (94%); limonene (96%) and α-humulene (96%); myristicin and aromadendrene (97%); α-pinene and β-pinene (98%); linalool, 1,8-cineole, β-caryophyllene, eugenol, pentadecane, heptadecane and tetradecanol (99.0%) were all purchased from Sigma-Aldrich (Saint Louis, MO, USA).
Dillapiole, asaricin, and safrole, all with a purity higher than 99.7%, were obtained via fractional distillation, as described in Section 4.2.