Human e selectin fc

Human E-selectin/Fc, human ICAM-1/Fc and human CXCL12 were from R&D Systems (R&D Systems, Minneapolis, MN, USA); fluorescein isothiocyanate (FITC) goat secondary antibody to mouse was from Sigma (Sigma-Aldrich, St. Louis, MO, USA); KIM127 mouse monoclonal antibody was from American Type Culture Collection (ATCC, Rockville, MD, USA); 327C and A mouse monoclonal antibodies were kindly provided by dr. Kristine Kikly (Eli Lilly and Co., Indianapolis, IN, USA); Tyrphostin AG490 and rabbit polyclonal anti-actin antibody were from Sigma; rabbit monoclonal anti-JAK2 (D2E12), was from Cell Signaling Technology (Danvers, MA, USA); siRNAs (ON-TARGETplus SMARTpool) were from Thermo Fisher Scientific (Fremont, CA, USA).

Human E-selectin/Fc, human ICAM-1/Fc, human VCAM-1/Fc and human CXCL12 were from R&D Systems (R&D Systems, Minneapolis, MN, USA); goat anti-human IgM was from SouthernBiotech (SouthernBiotech, Birmingham, AL, USA); fluorescein isothiocyanate (FITC) goat secondary antibody to mouse was from Sigma (Sigma-Aldrich, St. Louis, MO, USA); KIM127 mouse monoclonal antibody was from American Type Culture Collection (ATCC, Rockville, MD, USA); 327A mouse monoclonal antibody was kindly provided by Dr. Kristine Kikly (Eli Lilly and Co., Indianapolis, IN, USA); goat F(ab’)₂ anti-human IgM was from Southern Biotech (Southern Biotech, Birmingham, AL, USA); Tyrphostin and rabbit polyclonal anti-actin antibody were from Sigma; Ibrutinib was from Selleck Chemicals (Selleck Chemicals, Houston, TX, USA); PE mouse anti-BTK (pY223) antibody was from BD Biosciences (BD Biosciences, San Jose, CA, USA); rabbit monoclonal anti-JAK2 (D2E12) was from Cell Signaling Technology (Danvers, MA, USA); siRNAs (ON-TARGETplus SMARTpool) were from Thermo Fisher Scientific (Fremont, CA, USA).