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Ld c apochromat 1.1 w

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The LD C-Apochromat 1.1 W is a high-performance objective lens designed for Zeiss microscopes. It features a numerical aperture of 1.1 and a working distance of 0.6 mm, making it suitable for a wide range of high-resolution imaging applications.

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Lab products found in correlation

Lsm 710, by Zeiss (4 mentions) Ec epiplan neofluar 10x 0, by Zeiss (2 mentions) Zen 2012 sp5, by Zeiss (1 mentions)

Spelling variants of this product

C-Apochromat (80 citations) LD C-Apochromat (18 citations) LD C-Apochromat 40×/1.1 W Korr objective lens (4 citations) LD C-Apochromat 40×/1.1 NA W Corr M27 (3 citations) LD C-Apochromat 63x/1.15 W Corr M27 objective (2 citations) LD C-Apochromat 40×/1.1 W Corr objective (2 citations) LD C-Apochromat 40x/1.1 W Corr M27 objective (2 citations) LD C-Apochromat 40x/1.1 W Korr objective (2 citations)

4 protocols using ld c apochromat 1.1 w

1

Imaging Embryo Development with Confocal Microscopy

Cited 1 time
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Embryos at the ten-somite stage were mounted in 0.8% low-melting agarose and imaged at 25 °C using a laser scanning confocal microscope (LSM 710, Carl Zeiss) running the software Zen 2012 sp5 (Carl Zeiss). Confocal images of the region of interest in ubiquitous or mosaic membrane-labelled embryos were taken either at 2.5 s intervals using a ×40 water immersion objective (LD C-Apochromat 1.1 W, Carl Zeiss) or through a 3D timelapse with 1.0 µm optical sections every 16 s using a ×25 water immersion objective (LD C-Apochromat 1.1 W, Carl Zeiss). Imaging of ferrofluid droplets in the embryo was done as previously described28 (link). To visualize myosin and actin dynamics at the cell–cell contact over time, confocal images of Tg(actb2:MA-mCherry2)hm29 × Tg(actb2:myl12.1-EGFP) and Tg(actb2:MA-mCherry2)hm29 × Tg(actb1:GFP-Has UTRN)e116) double transgenic embryos, respectively, were taken in the region of interest at 2 s intervals using a ×40 water immersion objective. Ferrofluid droplets were labelled using a custom-synthesized fluorinated rhodamine dye58 (link), which was diluted in the ferrofluid oil at a final concentration of 37 μM.
Mongera A., Pochitaloff M., Gustafson H.J., Stooke-Vaughan G.A., Rowghanian P., Kim S, & Campàs O. (2022). Mechanics of the cellular microenvironment as probed by cells in vivo during zebrafish presomitic mesoderm differentiation. Nature Materials, 22(1), 135-143.
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2

Time-lapse 3D Confocal Imaging of Double Emulsion Droplets

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3D confocal timelapses of double emulsion droplets were acquired on a Zeiss LSM710 laser scanning confocal microscope with a 40x water immersion objective (LD C-Apochromat 1.1 W, Carl Zeiss Inc.) at room temperature.
Vian A., Pochitaloff M., Yen S.T., Kim S., Pollock J., Liu Y., Sletten E.M, & Campàs O. (2023). In situ quantification of osmotic pressure within living embryonic tissues. Nature Communications, 14, 7023.
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3

Live Imaging of Ferrofluid Labeling in Embryos

Cited 1 time
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Embryos were mounted for imaging in 0.8% low-melting agarose and imaged at 25 °C using a laser scanning confocal (LSM 710, Carl Zeiss Inc.). Images were taken every at 2.5 – 5 s (0.3 – 0.5 s) temporal resolution using a 40x water immersion objective (LD C-Apochromat 1.1W, Carl Zeiss Inc.) or a 10x air objective (EC Epiplan-Neofluar 10x 0.25, Carl Zeiss Inc.). Imaging of ferrofluid droplets in the embryo was done as previously reported20 (link). Ferrofluid droplets were fluorescently labelled using a custom-synthesized fluorinated Rhodamine dye33 (link), which was dissolved in the fluorocarbon-based ferrofluid oil at a final concentration of 37 μM.
Mongera A., Rowghanian P., Gustafson H.J., Shelton E., Kealhofer D.A., Carn E.K., Serwane F., Lucio A.A., Giammona J, & Campàs O. (2018). A fluid-to-solid jamming transition underlies vertebrate body axis elongation. Nature, 561(7723), 401-405.
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4

Live Imaging of Ferrofluid Labeling in Embryos

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryos were mounted for imaging in 0.8% low-melting agarose and imaged at 25 °C using a laser scanning confocal (LSM 710, Carl Zeiss Inc.). Images were taken every at 2.5 – 5 s (0.3 – 0.5 s) temporal resolution using a 40x water immersion objective (LD C-Apochromat 1.1W, Carl Zeiss Inc.) or a 10x air objective (EC Epiplan-Neofluar 10x 0.25, Carl Zeiss Inc.). Imaging of ferrofluid droplets in the embryo was done as previously reported20 (link). Ferrofluid droplets were fluorescently labelled using a custom-synthesized fluorinated Rhodamine dye33 (link), which was dissolved in the fluorocarbon-based ferrofluid oil at a final concentration of 37 μM.
Mongera A., Rowghanian P., Gustafson H.J., Shelton E., Kealhofer D.A., Carn E.K., Serwane F., Lucio A.A., Giammona J, & Campàs O. (2018). A fluid-to-solid jamming transition underlies vertebrate body axis elongation. Nature, 561(7723), 401-405.
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