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Api 4000 triple quadrupole

Manufactured by AB Sciex
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The API 4000 is a triple quadrupole mass spectrometer system designed for analytical and quantitative applications. It features high sensitivity, selectivity, and precision for the detection and measurement of target analytes in complex samples.

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Lab products found in correlation

Analyst 1, by AB Sciex (2 mentions) Lc 20ad, by Shimadzu (1 mentions) Multiquant 2, by AB Sciex (1 mentions) Luna omega polar c18 column, by Phenomenex (1 mentions) Sil 20acxr autosampler, by Shimadzu (1 mentions) Security guard cartridge, by Phenomenex (1 mentions) Lc 20ad uflc xr pumps, by Shimadzu (1 mentions)

Spelling variants of this product

API 4000 (203 citations) API 4000 triple quadrupole mass spectrometer (83 citations) API 4000 triple quadrupole tandem mass spectrometer (4 citations) API 4000 QTRAP triple quadrupole mass spectrometer (4 citations) API 4000 triple quadrupole instrument (4 citations) API 4000 triple quadrupole system (2 citations)

4 protocols using api 4000 triple quadrupole

1

Quantitative Lipidomics of C. albicans

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Approximately 1.25 × 108C. albicans cells from a saturated YPD culture were inoculated into 100 ml Synthetic Medium (SM) and grown at 30 °C to an OD of 1.0. Cells were washed once in modified SM (25 mM KPO4 pH 7.0, with glucose omitted) and suspended to 5 OD600 units per ml in modified SM.
Labelling reactions were performed in 12-ml glass tubes with PTFE-lined caps (Corning) and containing 1 ml cell suspension (∼6 × 107 cells), 10 μl DMSO or 10 μl compound dissolved in DMSO, 12 μl 1M D-Glucose (U-13C6 glucose), and 10 μl 1 M (1,2-13C2) sodium acetate (Cambridge Isotope Laboratories, Inc). Before LC-MS analysis, dried lipid extracts were dissolved in 500 μl methanol, vortexed and sonicated for 10 min.
Squalene, ergosterol, lanosterol and zymosterol were quantified using the tandem mass spectrometer API4000 triple quadrupole (AB Sciex) with atmospheric pressure chemical ionization (APCI), interfaced to an Agilent LC.
Helliwell S.B., Karkare S., Bergdoll M., Rahier A., Leighton-Davis J.R., Fioretto C., Aust T., Filipuzzi I., Frederiksen M., Gounarides J., Hoepfner D., Hofmann A., Imbert P.E., Jeker R., Knochenmuss R., Krastel P., Margerit A., Memmert K., Miault C.V., Rao Movva N., Muller A., Naegeli H.U., Oberer L., Prindle V., Riedl R., Schuierer S., Sexton J.A., Tao J., Wagner T., Yin H., Zhang J., Roggo S., Reinker S, & Parker C.N. (2015). FR171456 is a specific inhibitor of mammalian NSDHL and yeast Erg26p. Nature Communications, 6, 8613.
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2

Quantification of Glycerol Trioleate by LC-MS/MS

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Chromatographic analysis was performed on Shimadzu LC20AD (Shimadzu Corporation, Kyoto, Japan), consisting of a binary pump solvent management system, an online degasser and an auto-sampler. Gimini C18 (4.6 × 50 mm, 5 μm; Phenomenex, Cheshire, UK) was used, and the column temperature was maintained at the room temperature. The mobile phase was composed of dichloromethane and acetonitrile (35:65, v/v), and the flow rate was set at 1.0 mL/min. The injection volume was 5 μL. Mass spectrometer (API 4000 triple quadrupole; AB Sciex, Framingham, MA, USA) equipped with an atmospheric pressure chemical ionization source was used. The analytic detection was operated in the positive ion mode using a multiple reaction monitoring approach at m/z transitions of 603.5 → 271.0 for glycerol trioleate and 271.0 → 74.2 for IS. Data were acquired and processed using Analyst 1.6.1 software (AB Sciex).
Zhang Y., Zhang L., Zhang Q., Zhang X., Zhang T, & Wang B. (2018). Enhanced gastric therapeutic effects of Brucea javanica oil and its gastroretentive drug delivery system compared to commercial products in pharmacokinetics study. Drug Design, Development and Therapy, 12, 535-544.
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3

Quantifying Cellular Energy Metabolites

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Following extraction with perchloric acid, concentrations of the high-energy phosphates adenosine, guanosine, uridine, and cytidine tri-, di- and monophosphates (ATP, ADP, AMP, GTP, GDP, GMP, UTP, UDP, UMP, CTP, CDP, CMP) and the cofactors nicotinamide dinucleotide (NAD+), NAD+ phosphate (NADP+), and flavin adenine dinucleotide (FAD) were measured on an API 4000 triple quadrupole (AB Sciex, Concord, ON, Canada) by high-performance liquid chromatography-mass spectrometry (HPLC-MS)16 (link).
Heischmann S., Dzieciatkowska M., Hansen K., Leibfritz D, & Christians U. (2017). The Immunosuppressant Mycophenolic Acid Alters Nucleotide and Lipid Metabolism in an Intestinal Cell Model. Scientific Reports, 7, 45088.
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4

HPLC-MS/MS Quantification of Analytes

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The HPLC system was a SIL-20AC XR auto-sampler and LC-20AD UFLC XR pumps (Shimadzu, Tokyo, Japan). The mobile phases (MP) consisted of ultrapure water with 0.1% HCOOH (phase A) and methanol/isopropanol (9:1, v/v) with 0.1% HCOOH (phase B). The chromatographic separation was obtained on the Luna Omega Polar C18 column (3 μM, 100 Å, 50 x 2.1 mm) coupled with a Security Guard Cartridge (Polar, C18, 4 x 2.0 mm), both provided by Phenomenex (Castel Maggiore (BO), Italy). The mass spectrometry system used for the detection was an API 4000 triple quadrupole (AB SCIEX, Massachusetts, USA) with a TurboIonSpray source operating in positive ion mode. To optimize source and compound dependent parameters, solutions of each analyte at the concentration of 100 ng/mL were used with a flow rate of 20 μL/min. Data were processed with Analyst 1.6.3 and the quantification of the peaks was done with MultiQuant 2.1 (software package AB SCIEX).
Posocco B., Buzzo M., Poetto A.S., Orleni M., Gagno S., Zanchetta M., Iacuzzi V., Guardascione M., Puglisi F., Basile D., Pelizzari G., Marangon E, & Toffoli G. (2020). Simultaneous quantification of palbociclib, ribociclib and letrozole in human plasma by a new LC-MS/MS method for clinical application. PLoS ONE, 15(2), e0228822.
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