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Mm00845983 s1

Manufactured by Thermo Fisher Scientific
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Mm00845983_s1 is a pre-designed TaqMan Gene Expression Assay from Thermo Fisher Scientific. It is a ready-to-use solution for the detection and quantification of a specific target gene in mouse samples using real-time PCR technology. The assay includes a pair of gene-specific primers and a fluorescent probe that enables the precise measurement of gene expression levels.

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Lab products found in correlation

Mouse brain matrix, by Zivic Instruments (2 mentions) Taqman rna to cttm 1 step kit, by Thermo Fisher Scientific (2 mentions) Mm00447557 m1, by Thermo Fisher Scientific (2 mentions) Abi steponeplus real time pcr system, by Thermo Fisher Scientific (2 mentions)

2 protocols using mm00845983 s1

1

Quantifying Adrenergic Receptor 2A mRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Locus coeruleus adra2a mRNA levels were determined from adra2a KD and scramble AAV-injected mice by real time (quantitative, q)-PCR (n=4), using a Taqman® RNA-to-CTTM1-Step kit (Applied Biosystems, Foster City, USA). Briefly, brains were removed and 1-mm tissue punches were collected using 1-mm interval mouse brain matrix (Zivic Instruments, Pittsburgh, PA, USA) and 1-mm core diameter hollow needles. Total RNA was extracted from frozen tissues using TRIzol. qPCR was performed on an ABI StepOne Plus Real Time PCR system (Applied Biosystems, Foster City, USA) using the adra2a primers (Applied Biosystems, Mm00845983_s1, described previously43 . Data were evaluated with SDS 2.1 software, using the Comparative CT method (ΔΔCT) to measure gene expression. Relative expression of the adra2a mRNA was determined by comparing AAV-shRNA mRNA levels in the knockdown group to those in AAV-scramble, mice and normalized to expression of tyrosine hydroxylase (TH) mRNA (TH primers were:Applied Biosystems, Mm00447557_m1).
Zhang Z., Ferretti V., Güntan İ., Moro A., Steinberg E.A., Ye Z., Zecharia A.Y., Yu X., Vyssotski A.L., Brickley S.G., Yustos R., Pillidge Z.E., Harding E.C., Wisden W, & Franks N.P. (2015). Neuronal ensembles sufficient for recovery sleep and the sedative actions of α2 adrenergic agonists. Nature neuroscience, 18(4), 553-561.
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2

Quantifying Adrenergic Receptor 2A mRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Locus coeruleus adra2a mRNA levels were determined from adra2a KD and scramble AAV-injected mice by real time (quantitative, q)-PCR (n=4), using a Taqman® RNA-to-CTTM1-Step kit (Applied Biosystems, Foster City, USA). Briefly, brains were removed and 1-mm tissue punches were collected using 1-mm interval mouse brain matrix (Zivic Instruments, Pittsburgh, PA, USA) and 1-mm core diameter hollow needles. Total RNA was extracted from frozen tissues using TRIzol. qPCR was performed on an ABI StepOne Plus Real Time PCR system (Applied Biosystems, Foster City, USA) using the adra2a primers (Applied Biosystems, Mm00845983_s1, described previously43 . Data were evaluated with SDS 2.1 software, using the Comparative CT method (ΔΔCT) to measure gene expression. Relative expression of the adra2a mRNA was determined by comparing AAV-shRNA mRNA levels in the knockdown group to those in AAV-scramble, mice and normalized to expression of tyrosine hydroxylase (TH) mRNA (TH primers were:Applied Biosystems, Mm00447557_m1).
Zhang Z., Ferretti V., Güntan İ., Moro A., Steinberg E.A., Ye Z., Zecharia A.Y., Yu X., Vyssotski A.L., Brickley S.G., Yustos R., Pillidge Z.E., Harding E.C., Wisden W, & Franks N.P. (2015). Neuronal ensembles sufficient for recovery sleep and the sedative actions of α2 adrenergic agonists. Nature neuroscience, 18(4), 553-561.
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