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2 protocols using sc 68376

1

Mapping Human Connexin 30.2/31.3 in IMA

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Sections of internal mammary artery were prepared using standard methods. We performed immunohistochemistry using a rabbit polyclonal antibody [sc-68376, described as “connexin 29 Antibody (H-86)”: Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA] raised against an epitope corresponding to amino acids 194–279 of NP_853516.1 mapping at the C-terminus of human connexin 30.2/31.3 (NP_853516.1; 86/86 Identities, 86/86 Positives, 0/86 Gaps), to identify the presence and location of human gap junction gamma-3 protein.
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2

Cell Culture Reagents and Inhibitors

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AG-205, nomegestrol acetate (NMGA), cell culture media and all supplements were from Sigma-Aldrich (St. Louis, USA). Ponceau S, ethanol, aprotinin, leupeptin, pepstatin and pefabloc were from Carl Roth (Karlsruhe, Germany). 3-isobutyl-1methylxanthine (IBMX), actinomycin D, calcein-AM, H-89 dihydrochloride (H89), KT5720, Rp-8-Br-cAMP, mifepristone (RU 486), PSC833, Ro 20-1724 and SC68376 were from Santa Cruz Biotechnology (Heidelberg, Germany). Bordetella pertussis toxin (PTX) was from Enzo Life Sciences (Farmingdale, USA). PD98059 was from Calbiochem (San Diego, USA). Polyvinylidene difluoride (PVDF) membranes were from GE Healthcare (Little Chalfont, UK). DharmaFECT4 Transfection Reagent was from Dharmacon (Lafayette, USA). DMSO was from Merck (Darmstadt, Germany).
All other chemicals were of analytical grade purity.
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