Alexa fluor plus 488 conjugated anti mouse igg

We used the following antibodies: rabbit anti pAKT-S473, pAKT-T308, AKT, pERK1/2, ERK1/2, WAVE3 (Cell Signaling Technology); rabbit anti pSMAD3 and SMAD3 (Abcam); mouse anti-GFP (Clontech) (1:1000); goat horseradish-peroxidase-conjugated anti-mouse IgG and goat horseradish-peroxidase-conjugated anti-rabbit IgG (Calbiochem) (1:2000); mouse monoclonal anti-Actin (Sigma) (1:5000); and Alexa-Fluor-Plus-594-conjugated anti-rabbit IgG and Alexa-Fluor-Plus-488-conjugated anti-mouse IgG (Invitrogen). Vecta-shield with DAPI was from Vector Laboratories. Gel electrophoresis reagents were from Bio-Rad.

The following primary antibodies were from Cell Signaling Technology: Rabbit anti α-Tubilin (1:1000), mouse anti-p53 (1:1000) and rabbit anti-p53 (1:1000 for WB and 1:500 for immunofluorescence). Mouse monoclonal anti-Kindlin-2, clone 3A3 (1:2000 for WB and 1:500 for immunofluorescence) was from Millipore; rabbit polyclonal anti SerpinB2 (1:1000) and rabbit polyclonal anti Lamin B1 (1:3000) were from Abcam. Goat horseradish peroxidase-conjugated anti-mouse IgG (1:2000) and goat horseradish peroxidase-conjugated anti-rabbit IgG (1:2000) were from Calbiochem. Mouse monoclonal anti-Actin (1:5000) was from Sigma. Alexa Fluor Plus 594-conjugated anti-Rabbit IgG and Alexa Fluor Plus 488-conjugated anti-mouse IgG were from Invitrogen. Vecta-shield with 4′,6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories. Gel electrophoresis reagents were from Bio-Rad.