hCSs derived from five hiPS cell lines (4 individuals) were used for low O2 exposure in a total of 9 independent experiments. At 74–78 days of in vitro differentiation, hCS maintained in 21% O2 and 5% CO2, were transferred to a C-chamber hypoxia sub-chamber (Biospherix) in neural media (without growth factors) for 48 hours. The media was previously equilibrated for ~16 hours at <1% O2, 5% CO2, 37°C. The level of O2 was controlled using a Proox 110 Compact Oxygen Controller (Biospherix) and a mixed CO2/N2 compressed gas source. After 48 hours, hCS were immediately collected for analyses or transferred to an incubator with 21% O2 and 5% CO2 for another 72 hours (re-oxygenation condition).
Proox 110 compact oxygen controller
Manufactured by Biospherix
The Proox 110 Compact Oxygen Controller is a lab equipment device that provides precise control and monitoring of oxygen levels. It measures and regulates oxygen concentration within a defined environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using proox 110 compact oxygen controller
1
Hypoxia Exposure of hiPS-derived hCSs
2
Hypoxia Exposure of hiPS-derived hCSs
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hCSs derived from five hiPS cell lines (4 individuals) were used for low O2 exposure in a total of 9 independent experiments. At 74–78 days of in vitro differentiation, hCS maintained in 21% O2 and 5% CO2, were transferred to a C-chamber hypoxia sub-chamber (Biospherix) in neural media (without growth factors) for 48 hours. The media was previously equilibrated for ~16 hours at <1% O2, 5% CO2, 37°C. The level of O2 was controlled using a Proox 110 Compact Oxygen Controller (Biospherix) and a mixed CO2/N2 compressed gas source. After 48 hours, hCS were immediately collected for analyses or transferred to an incubator with 21% O2 and 5% CO2 for another 72 hours (re-oxygenation condition).
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