To extract the total protein in cells and tissues, RIPA lysis buffer was employed. Protein was quantified in each group using the BCA kit (Biosharp, Anhui, China) and subsequently mixed with sodium dodecyl sulfate polyacrylamide gel electrophoresis buffer; the protein was adsorbed on the polyvinylidene fluoride membrane. Then, the membranes were blocked in 5% bovine serum albumin (BSA) for 1 hour. ASC (10500-1-AP; Proteintech, Rosemont, IL, USA), cleaved caspase-1 (24232; CST, Danvers, MA, USA), GSDMD-N (ab215203; Abcam, Cambridge, UK), NLRP3 (19771-1-AP; Proteintech), IL-18 (10663-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), PTEN (9188; CST), and GAPDH (AB9485; Abcam) primary antibodies were used for overnight incubation. We then incubated horseradish peroxidase (HRP)-labeled secondary antibody IgG-HRP (BL003A; BioSharp). Exposure was performed using an ECL chemiluminescence substrate (Biosharp). To measure protein expressions, GAPDH was used as an internal reference.
Nlrp3 19771 1 ap
Manufactured by Proteintech
Sourced in United Kingdom
NLRP3 (19771-1-AP) is a primary antibody product offered by Proteintech. It is designed to detect the NLRP3 protein, which is a component of the NLRP3 inflammasome complex. The NLRP3 antibody can be used in various research applications involving the study of NLRP3 and its role in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Il 18 10663 1 ap, by Proteintech (1 mentions)
Asc 10500 1 ap, by Proteintech (1 mentions)
Ecl chemiluminescence substrate, by Biosharp (1 mentions)
Il1β 16806 1 ap, by Proteintech (1 mentions)
Bca kit, by Biosharp (1 mentions)
Gapdh, by Abcam (1 mentions)
Ab179515, by Abcam (1 mentions)
Caspase 1 22915 1 ap, by Proteintech (1 mentions)
Mcc950 b7946, by Apexbio (1 mentions)
Ab16502, by Abcam (1 mentions)
Ab9722, by Abcam (1 mentions)
2 protocols using nlrp3 19771 1 ap
1
Protein Extraction and Western Blot Analysis
2
Investigating NLRP3 Inflammasome Activation in Kidney Injury
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AS-IV (purity ≥ 98%) and anti-klotho antibody (SAB3500604) and anti-gasdermin D (GSDMD) (SAB2108448) were obtained from Sigma-Aldrich (St. Louis, MO, USA). IL-18 (A115) and anti-GSDMD antibody (A20197) were purchased from ABclonal Technology (Wuhan, China). NLRP3 (19771-1-AP) and caspase-1 (22915-1-AP) were obtained from Proteintech (Wuhan, China). Rabbit anti-phospho-nuclear-factor kappa-light-chain-enhancer of activated B cell (NF-κB) p65 antibody (3033) was acquired from Cell Signaling Technology. Antibodies against (NF-κB) p65 (ab16502), acetyl-coenzyme A synthetase 2 (ACS2; ab47092), pro-caspase-1 (ab179515), and IL-1β (ab9722) were purchased from Abcam (Cambridge, UK). Nephrin antibody (sc-377246) was obtained from Santa Cruz Biotechnology (Shanghai, China). MCC950 (B7946) and Z-YVAD-FMK (YVAD; A8955) were procured from ApexBio Technology (USA). Pyrrolidinedithiocarbamate ammonium (PDTC; S3633) was acquired from Selleck Chemicals (USA). N-Acetyl-L-cysteine (NAC; S0077) was provided by Beyotime (Shanghai, China).
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