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5 protocols using carboxy methylcellulose sodium salt cmc na

1

Aurantio-obtusin Analysis in Urine

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Aurantio-obtusin (purity 98%) was purchased from Beijing Saibaicao Technology Co., Ltd. (Beijing, China). Acetonitrile (HPLC-grade) was acquired from Thermo Fisher Scientific Co., Ltd. (Shanghai, China). Formic acid (HPLC-grade) was obtained from MREDA Technology, Co., Ltd. (MREDA, USA). The ultra-pure water was prepared by the Milli-Q system (Millipore, Bedford, MA, USA). Carboxymethyl cellulose sodium salt (CMC-Na) was provided from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). All other reagents were analytical grade. Analysis of urine was performed on an ACQUITY UPLC system (Waters Technologies, USA) equipped with a Synapt G2-Si mass spectrometer.
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2

Simultaneous Quantification of Vonoprazan and Voriconazole

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Vonoprazan (purity > 98%) and voriconazole (purity > 98%) were purchased from the Beijing Sunflower Technology Development Co. Ltd. (Beijing, China). M-I was purchased from the WuXi PharmaTech Co. Ltd. (Jiangsu, China). Diazepam (internal standard (IS); purity > 98%) was purchased from the Tianjin KingYork Pharmaceutical Co. Ltd. (Tianjin, China). Acetonitrile and methanol were purchased from Merck Co. Ltd. (Darmstadt, Germany). Carboxy methylcellulose sodium salt (CMC-Na) was purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai China). Formic acid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained by using a Milli-Q water purification system (Millipore, Billerica, MA, USA). Rat liver microsomes (RLM) were prepared in our laboratory. All other chemicals and biologicals were of analytical grade or higher.
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3

Tofacitinib Metabolism Kinetics in Liver Microsomes

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Tofacitinib (purity >98%) was purchased from Beijing InnoChem Science & Technology Co., Ltd. Psoralen, isoPsoralen, psoralidin, bergapten, 8-methoxyPsoralen and xanthotoxol were purchased from Chengdu Must Bio-technology Co., Ltd.Midazolam (internal standard (IS); purity >98%) was purchased from the Tianjin King York Pharmaceutical Co. Ltd. (Tianjin, China). HLMs was purchased from Wuhan PrimeTox biomedicine technology Co., Ltd. (Wuhan, China). rCYP3A4 was gifted from Beijing Hospital. Acetonitrile and methanol were bought from Merck Co., Ltd. (Darmstadt, Germany). Carboxymethylcellulose sodium salt (CMC-Na) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai China). Formic acid was bought from Sigma-Aldrich (St. Louis, MO, United States). Ultrapure water was obtained using a Milli-Q water purification system (Millipore, Billerica, MA, United States). RLMs was prepared in our laboratory. All other chemicals and biological substances were of analytical grade or higher.
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4

Comprehensive Uric Acid Regulation Protocol

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Allopurinol, hypoxanthine, and potassium oxonate were procured from Sigma-Aldrich Corporation Co. Ltd (MO, USA). Uric acid (UA), blood urea nitrogen (BUN), xanthine oxidase (XOD), creatinine (CRE), interleukin-6 (IL-6), and interleukin-1β (IL-1β) assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (China). Carboxymethylcellulose sodium salt (CMC-Na) was purchased from Sinopharm Chemical Reagent Co., Ltd (China). Moreover, UPLC-grade methanol, acetonitrile, and ammonium acetate were supplied by the Tedia Company Inc. (Fairfield, USA). Water was prepared with the ultrapure water purifying system of Hitech Instruments Co., Ltd (China). All the incorporated chemicals were of analytical grade.
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5

Analytical Method for Three Drugs

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Dacomitinib (purity > 98%), poziotinib (purity > 98%) and enasidenib (IS purity > 98%) were purchased from the Beijing Sunflower and Technology Development Co. Ltd. (Beijing, China). Acetonitrile and methanol were purchased from Fisher Scientific Co. (Fair Lawn, NJ, USA). Formic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained from a Milli-Q water purification system (Millipore, Billerica, MA, USA). Carboxy methylcellulose sodium salt (CMC-Na) was from Sinopharm Chemical Reagent Co. Ltd (Shanghai China). Food was purchased from Shenyang Maohua Biotechnology Co. Ltd (Shenyang China). The reduced form of nicotinamide adenine dinucleotide phosphate was purchased from Roche Co. Ltd (Shanghai China). HLM were purchased from Corning Co. Ltd (Woburn, MA, USA). RLM were obtained from our laboratory. All other chemicals were of analytical grade or better.
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