Rf 5301 pc spectrofluorometer model f 2000

Fluorescence measurements were recorded using an RF-5301 PC Hitachi spectrofluorometer Model F-2000 (Hitachi, Tokyo, Japan) equipped with a 150 W Xenon lamp and a slit width of 5 nm. A 1.00 cm quartz cell and thermostatic cuvette holder were used for measurements. The absorption spectra were recorded on a double beam CARY 50-BIO UV–vis spectrophotometer (Varian, Australia) equipped with a 150 W Xenon lamp and a slit width of 5 nm. A 1.00 cm quartz cell was used for measurements. The pH of solution was measured with an Elico LI120 pH meter (Elico Ltd., India). Circular dichroism measurements were recorded on a Jasco J-715 spectropolarimeter (Tokyo, Japan) with a 0.2 cm quartz cell.

Fluorescence spectra were recorded using an RF-5301 PC Hitachi Spectrofluorometer Model F-2000 (Tokyo, Japan) with a 150 W Xenon lamp, a 1 cm quartz cell and thermostatic cuvette holder. The excitation and emission bandwidths were both 5 nm and the scan rate was 1200 nm/s. The temperature of the sample was maintained by recycling water throughout the experiment. Circular dichroism (CD) measurements were recorded on a Jasco J-715 spectropolarimeter (Tokyo, Japan) with a 0.2 cm quartz cell. The absorption spectra were recorded on a double beam CARY 50-BIO UV–visible spectrophotometer (Victoria, Australia) with a scan rate of 600 nm/min. Fourier transform infrared (FT-IR) spectroscopic measurements were made at room temperature on a Nicolet 5700 FT-IR spectrometer equipped with a germanium attenuated total reflection (ATR) accessory, a DTGS KBr detector and a KBr beam splitter. The pH measurements were performed with an Elico LI120 pH meter (Elico Ltd., India). Fluorescence lifetime measurements were carried out in an ISS', Chronos BH fluorescence lifetime spectrometer (ISS Inc, USA).