Dl threo β benzyloxyaspartic acid

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DL-threo-β-Benzyloxyaspartic acid is a chemical compound used in laboratory research. It functions as a glutamate transporter inhibitor.

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DL-threo-β-benzyloxyaspartic acid (TBOA) (6 citations) DL-threo-β-Benzyloxyaspartic acid (DL-t-BOA) (5 citations)

2 protocols using dl threo β benzyloxyaspartic acid

Pharmacological Agents in Neurophysiology

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Dyes and drugs were purchased from the following suppliers: (RS)-AMPA hydrobromide (AMPA), Kainate (KA), GYKI 52466 hydrochloride (GYKI), DL-threo-β-Benzyloxyaspartic acid (TBOA), NBQX disodium salt (NBQX), DL-AP5 sodium salt (APV), dynamin inhibitory peptide (QVPSRPNRAP), dynamin inhibitory peptide myristoylated (scrambled), dynamin inhibitory peptide myristoylated, cyclothiazide, mecamylamine, dhβe, and Dynasore (Tocris, Ellisville, MO, USA); N-Methyl-D-Aspartate (NMDA), tetrodotoxyn (TTX), Strychnine, Bicuculline and L-glutamate, atropine (Sigma, St Louis, MO); Fluoro-Gold (Fluorochrome, Inc., Denver, CO, USA); Lucifer Yellow (LY), Acridine Orange 10-Nonyl (AO), 6-methoxy-N-ethylquinolinium iodide (MEQ), and Florescien-5-isothiocyan (FITC) were from Invitrogen. Dynasore was dissolved in DMSO. When bath applied to the cord, the concentration of DMSO was less than 0.1%. All other drugs were dissolved in artificial cerebrospinal fluid (aCSF).

Falgairolle M, & O’Donovan M.J. (2015). Pharmacological Investigation of Fluoro-Gold Entry into Spinal Neurons. PLoS ONE, 10(6), e0131430.

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Pharmacological Tools for Synaptic Modulation

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t-ACPD, DHPG and ATP (Sigma) were applied by pressure-application through a patch pipette. Other agonists and blockers were applied through the bath perfusion. t-ACPD, 2-methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP); 2,3-dioxo−6-nitro−1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX); d-(−)−2-amino−5-phosphonopentanoic acid (D-APV), 6-imino−3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide (SR95531), 7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxylate ethyl ester (CPCCOEt), tetrodotoxin (TTX), dl-threo-β-benzyloxyaspartic acid (TBOA), (3S)-3-[[3-[[4-(trifluoromethyl)benzoyl]amino]phenyl]methoxy]-l-aspartic acid (TFB-TBOA), (S)−3,5-dihydroxyphenylglycine (DHPG) were purchased from Tocris or Abcam. We thank W. Fröstle for the kind gift of CGP55845A (3-[1-(S)-(3,4-dichlorophenyl)ethyl]amino−2(S)hydroxypropyl-P-benzyl-phosphonic acid).

Otsu Y., Couchman K., Lyons D.G., Collot M., Agarwal A., Mallet J.M., Pfrieger F.W., Bergles D.E, & Charpak S. (2014). Calcium dynamics in astrocyte processes during neurovascular coupling. Nature neuroscience, 18(2), 210-218.

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