Rabbit monoclonal anti α tubulin

Manufactured by Cell Signaling Technology

Rabbit monoclonal anti-α-tubulin is a primary antibody that specifically recognizes the α-tubulin protein. α-tubulin is a key component of microtubules, which are essential for various cellular processes such as cell division, intracellular transport, and cell motility.

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Lab products found in correlation

Ab19903, by Abcam (1 mentions) Ab117600, by Abcam (1 mentions) Af2805, by R&D Systems (1 mentions) Af1119, by R&D Systems (1 mentions) Af3264, by R&D Systems (1 mentions) Chemidoc touch, by Bio-Rad (1 mentions) Gradient sds page gel, by Bio-Rad (1 mentions) Image lab software, by Bio-Rad (1 mentions) Ab80508, by Abcam (1 mentions)

2 protocols using rabbit monoclonal anti α tubulin

Exosomal Protein Profiling by Western Blot

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Exosomes or whole cell lysates were loaded onto 4–20% gradient SDS-PAGE gels (Bio-Rad) and transferred onto nitrocellulose membranes. The membranes were blocked with Block-One^TM blocking reagent (Nacalai Tesque) and then incubated with primary antibodies using Can Get Signal^TM solution 1 (TOYOBO) overnight at 4 °C and followed by incubation with secondary antibodies conjugated with HRP using Can Get Signal^TM solution 2 (TOYOBO) for 60 minutes at room temperature. The following primary antibodies were used: goat polyclonal anti-adiponectin (AF1119, R&D); goat polyclonal anti-T-cadherin (AF3264, R&D); rabbit monoclonal anti-α-tubulin (11H10, Cell Signaling); rat monoclonal anti-mouse CD63 (clone R5G2, MBL); rabbit polyclonal anti-syntenin (ab19903, abcam); rabbit polyclonal anti-Tsg101 (ab125611, abcam); goat polyclonal anti-MFG-E8 (AF2805, R&D); mouse monoclonal anti-Alix (ab117600, abcam); rabbit polyclonal anti-Nox2/gp91 phox (ab80508, abcam). CD63 was detected under non-reducing conditions. Chemiluminescence signals developed with Chemi-Lumi One Super^TM (Nacalai Tesque) were visualized by ChemiDoc Touch^TM and quantitated using Image Lab software (Bio-Rad).

Tanaka Y., Kita S., Nishizawa H., Fukuda S., Fujishima Y., Obata Y., Nagao H., Masuda S., Nakamura Y., Shimizu Y., Mineo R., Natsukawa T., Funahashi T., Ranscht B., Fukada S.I., Maeda N, & Shimomura I. (2019). Adiponectin promotes muscle regeneration through binding to T-cadherin. Scientific Reports, 9, 16.

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Adiponectin and T-cadherin Immunodetection

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The following primary antibodies were used: goat polyclonal anti-mouse adiponectin (AF1119, R and D), goat polyclonal anti-human adiponectin (AF1065, R and D), goat polyclonal anti-T-cadherin (AF3264, R and D), rabbit monoclonal anti-α-tubulin (11H10, Cell Signaling), rat monoclonal anti-PA-tag (human podoplanin PLAG sequence) (012–25863, FUJIFILM), and rabbit monoclonal anti-GPADH (14C10, Cell Signaling Technology).

Kita S., Fukuda S., Maeda N, & Shimomura I. (2019). Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin. eLife, 8, e48675.

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