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4 protocols using k2so4

1

Preparation of Metal-Chitosan Composites

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l-Valine (Val), phosphoric acid, NaOH,
FeCl2, FeCl3, ethanol, K2SO4, K3PO4, K2CO3, Hg(NO3)2, AgNO3, ZnCl2, CuSO4, CdCl2, BaCl2, Pb(NO3)2, MnCl2, CaCl2, MgCl2, Cr2(SO4)3, and K2Cr2O7 were bought from Sinopharm Chemical Reagent Co. Ltd.
(Beijing, China).
Poly(ethylene oxide) (average Mw 900 kDa)
and Chitosan (average Mw 200 kDa) were
obtained from Sigma-Aldrich. Dialysis tubing with a flat diameter
of 28 mm and a molecular weight cutoff (MWCO) of 1 kDa were provided
by Biotopped (Viskase). Deionized water with a resistivity of 18.2
MΩ was provided by a GWA-UN water purification system. All materials
and chemicals were of analytical grade and used as received without
further purification.
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2

Phosphate-Based Polymer Admixtures in Cement

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Industrial-grade isobutyl alcohol polyoxyethylene ether (IPEG) with a molecular weight of 2400 (average polymerization degree: ~54), and 2-(acryloyloxy) ethyl phosphate (MOEP) and 5-hydroxypentyl prop-2-enoate phosphate (HPEP), were manufactured by Liaoning Kelong Fine Chemical Company (Liaoyang, Liaoning Province, China), and Guangzhou Kinde Chemical Materials Company (Guangzhou, Guangdong Province, China), respectively. Analytical grade acrylic acid (AA), 2-(Dimethylamino)ethyl methacrylate (DMAEMA), Maleic anhydride (MA), N,N′-Methylenebisacrylamide (MBA), potassium persulfate (KPS), mercapto acetic acid (TGA), CaSO4·2H2O, KOH, K2SO4, Na2SO4, and ascorbic acid (VC) were from Sinopharm Chemical Company (Shanghai, China). The schematic drawings of the unsaturated phosphate monomers are exhibited in Figure 1. The phosphate monoester, phosphate diester, and free phosphoric acid of unsaturated phosphate ester are shown in Table 1.
Ordinary Portland cement classified as P Ⅱ 52.5 was purchased from Sichuan E-Sheng Cement Group Co., Ltd. Chemical (Emei, Sichuan Province, China) compositions of cement are tabulated in Table 2.
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3

Synthesis of Inorganic Compounds

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Folic acid (AR), NaOH (AR), H2O2 (AR), NaH2PO4 (AR), NH4F (AR), KCl (AR), NaBr (AR), K2SO4 (AR) and NaNO3 (AR) were provided by Sinopharm Chemical Reagent Co. Deionized water was generated by the Hitech-K flow water purification system.
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4

Optimizing Lactic Acid Bacteria Growth

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AP was provided by Kangyuan Biotechnology Co. Ltd (Zibo, China). Peptone, yeast extract, beef extract, agar, and Man Rogosa Sharpe (MRS) medium were obtained from AOBOX (Beijing, China). Glucose, K 2 HPO 4 , MgSO 4 •7H 2 O, CuSO 4 , K 2 SO 4 , H 2 SO 4 were obtained from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Distilled water was prepared in the laboratory.
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