Reticulin silver plating kit

Manufactured by Merck Group

Sourced in France

The Reticulin silver plating kit is a specialized laboratory equipment designed for the visualization and staining of reticular fibers in histological samples. It provides a reliable and consistent method for the detection and analysis of these important connective tissue components.

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2 protocols using reticulin silver plating kit

Comprehensive Liver Histopathology Analysis

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Immunohistochemical staining was performed on paraffin-embedded liver sections (5 microns) for immune cell infiltration [Ly6G (1:200), F4/80 (1:50), & CD3 (1:200)], cell proliferation [PCNA (1:2000), & Ki67 (1:250)], hepatobiliary tract (Cytokeratin 19; 1:100) and HCC markers (glypican-3; 1:200). Source of all antibodies are incorporated in key resources table. Immunoreactivity was revealed by using Vectastain Elite ABC kit and peroxidase substrate kit (Vector Laboratories, Burlingame, CA). Similar exposure time for each section was used to acquire the image. Liver reticular fiber was stained using the reticulin silver plating kit (EMD Millipore, MA) according to the manufacturer’s protocol.

Singh V., Yeoh B.S., Chassaing B., Xiao X., Saha P., Olvera R.A., Lapek JD J.r., Zhang L., Wang W.B., Hao S., Flythe M.D., Gonzalez D.J., Cani P.D., Conejo-Garcia J.R., Xiong N., Kennett M.J., Joe B., Patterson A.D., Gewirtz A.T, & Vijay-Kumar M. (2018). Dysregulated Microbial Fermentation of Soluble Fiber Induces Cholestatic Liver Cancer. Cell, 175(3), 679-694.e22.

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Immunohistochemical Profiling of Tissue Samples

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Unstained 3-μm-thick slides of formalin-fixed, paraffin-embedded tissues were obtained and submitted for immunostaining with an automated stainer (Dako Omnis, Glostrup, Denmark). The following primary antibodies were used: CD56 (pre-diluted, clone 123C3, Dako, Glostrup, Denmark), Glial Fibrillary Acidic Protein (GFAP) (1:200, clone 6F2, Dako, Glostrup, Denmark), Olig2 (1:500, clone OLIG2, Sigma-Aldrich, Saint-Louis, USA), vimentin (1:800, clone V9, Dako, Glostrup, Denmark), neurofilament (1:100, clone NF70, Dako, Glostrup, Denmark), NeuN (1:1000, clone A60, Sigma-Aldrich, Saint-Louis, USA), synaptophysin (1:150, clone Synap, Dako, Glostrup, Denmark), EMA (1:200, clone GM008, Dako, Glostrup, Denmark), CK18 (1:200, clone 6F2, Dako, Glostrup, Denmark), smooth muscle actin (1:4000, clone 1A4, Dako, Glostrup, Denmark), NFκB (1:6000, clone D14E12, Cell Signaling Technology, Danvers, USA), L1CAM (1:500, clone UJ127.11, Sigma-Aldrich, Saint-Louis, USA), and Ki-67 (1:200, clone MIB-1, Dako, Glostrup, Denmark). Reticulin staining was performed using the Reticulin silver plating kit according to Gordon & Sweets (Merck Millipore, Guyancourt, France). External positive and negative controls were used for all antibodies and staining.

Tauziède-Espariat A., Siegfried A., Nicaise Y., Kergrohen T., Sievers P., Vasiljevic A., Roux A., Dezamis E., Benevello C., Machet M.C., Michalak S., Puiseux C., Llamas-Gutierrez F., Leblond P., Bourdeaut F., Grill J., Dufour C., Guerrini-Rousseau L., Abbou S., Dangouloff-Ros V., Boddaert N., Saffroy R., Hasty L., Wahler E., Pagès M., Andreiuolo F., Lechapt E., Chrétien F., Blauwblomme T., Beccaria K., Pallud J., Puget S., Uro-Coste E, & Varlet P. (2021). Supratentorial non-RELA, ZFTA-fused ependymomas: a comprehensive phenotype genotype correlation highlighting the number of zinc fingers in ZFTA-NCOA1/2 fusions. Acta Neuropathologica Communications, 9, 135.

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