Formalin (4%) fixed duodenum and colon tissue samples were embedded in paraffin, and a 4 μm section of each was obtained on a glass slide. These sections were deparaffinized and incubated with anti-HIV p24 (clone: Kal-1, Dako), anti–SARS-CoV-2 nucleoprotein (clone: 40143-T-62, Sino Biological) anti-ACE2 (clone: ab15348; Abcam), anti-TMPRSS2 (clone: ab109131; Abcam), and anti-EpCAM (clone: ab71916; Abcam) followed by a secondary antibody incubation using the Opal 4-color manual IHC (PerkinElmer) as instructed by the manufacturer. For Opal fluorophores (PerkinElmer), FITC (product number FP1487001KT; PerkinElmer) was used for EpCAM and p24, Texas red (product number FP1488001KT; PerkinElmer) for ACE2, and Cy5 (product number FP1497001KT; PerkinElmer) for TMPRSS2 and SARS-CoV-2 nucleoprotein signal generation. DAPI was used as the nuclear counterstain. The sections were mounted with the Fluorescence Mounting Medium (catalog number S302380-2; Agilent Technologies) and cover-slipped, and the edges were sealed with nail polish. The slides were stored at 2°C–8°C until images were acquired.
Anti sars cov 2 nucleoprotein
Manufactured by Sino Biological
The anti-SARS-CoV-2 nucleoprotein is a laboratory reagent used for the detection and study of the SARS-CoV-2 virus. It is a recombinant protein that specifically binds to the nucleoprotein of the SARS-CoV-2 virus, which is a key component of the viral structure. This reagent can be used in various immunoassays and research applications to identify and quantify the presence of the SARS-CoV-2 virus.
Automatically generated - may contain errors
Lab products found in correlation
Anti epcam, by Abcam (1 mentions)
Opal fluorophores, by PerkinElmer (1 mentions)
Anti tmprss2, by Abcam (1 mentions)
Texas red, by PerkinElmer (1 mentions)
Fluorescein isothiocyanate (fitc), by PerkinElmer (1 mentions)
Anti ace2, by Abcam (1 mentions)
Fluorescence mounting medium, by Agilent Technologies (1 mentions)
Supersignal west pico chemiluminescent substrate, by Bio-Rad (1 mentions)
Anti ripk1, by Cell Signaling Technology (1 mentions)
Anti strep tag 2, by Abcam (1 mentions)
Anti tubulin, by Transgene (1 mentions)
Anti p hripk1, by Cell Signaling Technology (1 mentions)
Anti actin, by Transgene (1 mentions)
Ipfl10100, by Merck Group (1 mentions)
Anti myc, by Merck Group (1 mentions)
Anti egfr, by Cell Signaling Technology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Rnascope probe 5 ncov2019 s, by Advanced Cell Diagnostics (1 mentions)
Taqman 2019 ncov assay kit v2, by Thermo Fisher Scientific (1 mentions)
Allplex 2019 ncov assay, by Seegene (1 mentions)
3 protocols using anti sars cov 2 nucleoprotein
1
Multimodal IHC for COVID-19 Markers
2
Immunoblotting for SARS-CoV-2 Proteins
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Cell lysates were boiled in 2× Laemmli sample buffer containing 10% βME (Sigma, M3148). Cell lysates were analyzed by SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, IPFL10100). After blocking with 5% BSA in TBS buffer containing 0.05% Tween 20, the blot was sequentially probed with primary antibodies and the horseradish peroxidase (HRP)-conjugated secondary antibody. Protein bands were detected by SuperSignal West Pico Chemiluminescent substrate (Bio-Rad). The following antibodies were used: anti-ACE2 (Abcam, ab108209), anti-SARS-CoV-2 nucleoprotein (Sino Biological, 40588-T62), anti-p-hRIPK1 (Cell Signaling Technology, 65746), anti-RIPK1 (Cell Signaling Technology, 3493), anti-EGFR (Cell Signaling Technology, 4267), anti-NSP12(Cell Signaling Technology), anti-Flag (Sigma, F1804), anti-Myc (Sigma, C3956), anti-Strep tag II (Abcam, ab76949), anti-Actin (TransGen Biotech, HC201) and anti-Tubulin (TransGen Biotech, HC101).
3
Placental SARS-CoV-2 Infection Analysis
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A total of 198 placentas delivered from SARS-CoV-2-positive pregnant women were submitted for conventional pathological analysis. Sampling was performed according to recommendations of the Amsterdam Consensus for placental sampling and report statements [10 (link)]. All placentas were immunohistochemically investigated for the presence of SARS-CoV-2 with the anti-SARS-CoV-2 nucleoprotein (Sino Biological, Wayne, PA) according to manufacturer's instructions. Immunohistochemically positive cases were further analyzed by in situ hybridization (ISH) with the RNAscope-ProbeV-nCoV2019-S (Advanced Cell Diagnostics, Hayward, CA). Thirty-three placentas were investigated for SARS-CoV-2 infection through retrotranscriptase PCR (RT-PCR). Fresh tissue samples and/or swabs were investigated by RT-PCR for SARS-CoV-2 with either the Allplex 2019-nCoV Assay (Seegene, Seoul, South Korea) or the Taqman 2019-nCoV Assay kit v.2 (ThermoFisher Scientific, Waltham, MA, US). For formaldehyde-fixed, paraffin embedded samples, RNA was obtained by High Pure FFPET extraction kit (Roche Diagnostics Gmbh) and further analyzed with CLART®COVID-19 (Genomica SAU) following the manufacturer's instructions. Clinical data were retrieved from the institutional records. Significance of association between qualitative variables was tested by Fisher's exact test with an α-risk of 0.05.
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