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Cardiophase hscrp assay

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The CardioPhase hsCRP assay is a quantitative in vitro diagnostic test used to measure high-sensitivity C-reactive protein (hsCRP) levels in human serum or plasma samples. hsCRP is a biomarker that can provide information about inflammation and cardiovascular health.

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Lab products found in correlation

Nephelometer, by Siemens (2 mentions) Liaison cmv igg 2, by DiaSorin (1 mentions) Liaison analyzer, by DiaSorin (1 mentions) Cobas e411 analyser, by Roche (1 mentions) Synchron lx20, by Beckman Coulter (1 mentions) Cobas mira plus, by Roche (1 mentions)

7 protocols using cardiophase hscrp assay

1

Serum Biomarkers in Disease Study

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Fasting whole blood was collected at the time of recruitment for all subjects, with serum and plasma isolated by centrifugation and stored at −80°C. Serum Hs-CRP measurement was done using the CardioPhase® hsCRP assay (Dade Behring Inc., Newark, USA) on the BN Systems, which measures Hs-CRP concentrations by means of particle enhanced immunonephelometry. The limit of detection of the assay was 0.175mg/L for sample dilution of 1:20 and the coefficient of variation was <10% for concentrations between 0.5mg/L and 62mg/L (BN Pro-spec System instruction manual).
Plasma was used at 1:4 dilution to measure 6 cytokine levels (IL6, IL8, IL1B, TNF-α, IL4 and IL10) using the BD™ Cytometric Bead Array (CBA) Human soluble Protein Flex set system. For both the CRP and cytokine measurements, all the samples were blinded and randomly distributed in analysis plates.
Basavaraju U., Shebl F., Palmer A., Berry S., Hold G., El-Omar E, & Rabkin C. (2015). Cytokine gene polymorphisms, cytokine levels and the risk of colorectal neoplasia in a screened population of Northeast Scotland. European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP), 24(4), 296-304.
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2

Comprehensive Metabolic and Immune Profiling

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Venous peripheral blood samples, collected after an overnight fast, underwent basal biochemical laboratory determinations. Serum, plasma, and polymorphonuclear leukocytes, were separated, aliquoted and stored frozen at -80 °C in the Biological Bank of INRCA until analysis. The blood haematocrit and haemoglobin concentration, were determined by automated blood analysers. Serum total cholesterol, HDL-cholesterol, triglycerides, were measured using an automatic analyser under conventional enzymatic methodology.
Glucose, creatinine and albumin levels were determined using standard laboratory methods.
C-reactive protein (CRP) levels were detected by CardioPhase hsCRP assay (Dade Behring Inc Deerfield, IL). Immunoglobulin G levels of CMV-specific antibodies were determined in plasma samples by the Liaison assay (LIAISON® CMV IgG II; Diasorin) according to the manufacturer's specifications and using the Liaison analyzer (DiaSorin, Italy).
Pesi B., Ferrero A., Grazi G.L., Cescon M., Russolillo N., Leo F., Boni L., Pinna A.D., Capussotti L, & Batignani G. (2015). Liver resection with thrombectomy as a treatment of hepatocellular carcinoma with major vascular invasion: results from a retrospective multicentric study. American journal of surgery, 210(1).
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3

Biomarker Measurement Protocols

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High-sensitivity C-reactive protein (hs-CRP) and interleukin-6 (IL-6) were measured in baseline serum samples using the CardioPhase® hs-CRP assay (Siemens Healthcare, Erlangen, Germany) on a Dimension Vista® 1500 system and the Elecsys® IL-6 assay (Roche Diagnostics, Rotkreuz, Switzerland) on a cobas® e 411 analyser, respectively.
Holstein K., Matysiak A., Witt L., Sievers B., Beckmann L., Haddad M., Renné T., Voigtlaender M, & Langer F. (2020). LPS-induced expression and release of monocyte tissue factor in patients with haemophilia. Annals of Hematology, 99(7), 1531-1542.
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4

Blood Lipid and Inflammatory Marker Analysis

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Blood samples were collected by ethylenediaminetetraacetic acid (EDTA) coated Vacutainer venipuncture just before the scan. Samples were kept at 4 °C and centrifuged at 4100 rpm for 10 minutes, and then stored at −80 °C until analysis, all within two hours. High-density lipoprotein cholesterol was measured using Synchron LX20 (Beckman Coulter). Low-density lipoprotein cholesterol was calculated using the Friedewald equation, or Cobas Mira Plus (Roche Diagnostics). High-sensitivity C‑reactive protein (hsCRP) was measured on BN ProSpec using the CardioPhase hsCRP assay (Siemens Diagnostics).
Versteylen M.O., Manca M., Joosen I.A., Schmidt D.E., Das M., Hofstra L., Crijns H.J., Biessen E.A, & Kietselaer B.L. (2016). CC chemokine ligands in patients presenting with stable chest pain: association with atherosclerosis and future cardiovascular events. Netherlands Heart Journal, 24(12), 722-729.
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5

Quantification of CRP and CXCL10 in Plasma

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Plasma samples were centrifuged at 20,000 x g for 10 minutes to remove brin or debris. Samples (100 µL) were then loaded undiluted and CRP levels measured using the Cardio Phase hsCRP assay on a Siemens Nephelometer calibrated with the Rheumatology Standard according to manufacturer's directions. The assay has a range of 0.1 -50 mg/L. Plasma levels of CXCL10 quanti cation CXCL10 levels were measured in the plasma of ZZ patients with liver disease and MM controls using an ELISA kit from R&D Systems according to manufacturer's instruction. Samples were diluted 1:2 in assay diluent before being applied to the assay plate for 2 hours incubation at room temperature. The plate was washed, Human IP-10 Conjugate added to each well, and the plate incubated for another 2 hours at room temperature. The plate was washed again and substrate solution added to each well. The plate was incubated for 30 minutes in the dark before adding stop solution. The optical density was measured using a SpectraMax spectrometer at 450 nm and the sample concentrations calculated based on the standard curve.
Khodayari N., Oshins R., Holliday S., Clark V., Xiao Q., Marek G., Mehrad B., & Brantly M. (2020). Alpha-1 antitrypsin deficient individuals have circulating extracellular vesicles with profibrogenic cargo.
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6

Quantification of CRP and CXCL10 in Plasma

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Plasma samples were centrifuged at 20,000 x g for 10 minutes to remove brin or debris. Samples (100 µL) were then loaded undiluted and CRP levels measured using the Cardio Phase hsCRP assay on a Siemens Nephelometer calibrated with the Rheumatology Standard according to manufacturer's directions. The assay has a range of 0.1 -50 mg/L. Plasma levels of CXCL10 quanti cation CXCL10 levels were measured in the plasma of ZZ patients with liver disease and MM controls using an ELISA kit from R&D Systems according to manufacturer's instruction. Samples were diluted 1:2 in assay diluent before being applied to the assay plate for 2 hours incubation at room temperature. The plate was washed, Human IP-10 Conjugate added to each well, and the plate incubated for another 2 hours at room temperature. The plate was washed again and substrate solution added to each well. The plate was incubated for 30 minutes in the dark before adding stop solution. The optical density was measured using a SpectraMax spectrometer at 450 nm and the sample concentrations calculated based on the standard curve.
Khodayari N., Oshins R., Holliday S., Clark V., Xiao Q., Marek G., Mehrad B., & Brantly M. (2020). Alpha-1 antitrypsin deficient individuals have circulating extracellular vesicles with profibrogenic cargo&nbsp;.
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7

Plasma hsCRP Quantification Protocol

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The lipid and laboratory safety parameters were measured by routine methods. The plasma hsCRP concentration was determined using an immunonephelometric method (Siemens Dade Behring CardioPhase hsCRP assay, Newark, DE, USA) on the Siemens BN ProSpec® System with the detection limit of 0.146 mg/L (the measurement range was 0.146–9.35 mg/L) and the inter-assay coefficients of variation of 2.5, 3.8 and 2.1 % at hsCRP concentrations of 0.500, 1.30 and 2.10 mg/L, respectively.
Ding C., Hu M., Wu Y.J, & Tomlinson B. (2015). Achievement of specified lipid and high-sensitivity C-reactive protein levels with two statins in Chinese patients with hypercholesterolaemia. Lipids in Health and Disease, 14, 107.
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