Cells were inoculated with 24-well plates and cultured overnight, then fixed with formaldehyde and permeabilized with 0.1% Triton X-100. Blocking cells with 3% normal sheep serum (ZSbio, Beijing, China), then cells will be incubated with primary antibodies (DHX9, 1:200; TGFβR1, 1:200) overnight at 4°C. Next day, after incubated the fluorophore-conjugated secondary antibody, cell nuclei were be labelled by Fluoroshield mounting medium with DAPI (Abcam). Fluorescence images are acquired by a multi-channel fluorescence microscope (Nikon, Japan).
Multi channel fluorescence microscope
Manufactured by Nikon
Sourced in Japan
The Multi-channel fluorescence microscope is a laboratory instrument used for the observation and analysis of fluorescently labeled samples. It employs multiple excitation and emission channels to simultaneously detect and differentiate various fluorescent probes within a single specimen.
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Lab products found in correlation
2 protocols using multi channel fluorescence microscope
1
Immunofluorescence Assay for Protein Localization
2
Immunofluorescence Staining of Cultured Cells
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Cells to be tested were inoculated with 24-well plates and cultured overnight, then fixed with formaldehyde at −20 °C for 20 min and permeabilized with 0.1% Triton X-100. Blocking cells with 3% normal sheep serum (ZSbio, Beijing, China) for 1 h at room temperature, then cells will be incubated with primary antibodies overnight at 4 °C. Next day, incubate the fluorophore-conjugated secondary antibody at room temperature. Fluoroshield mounting medium with DAPI (Abcam) was used for nuclei labeling. Fluorescence images are acquired by a multi-channel fluorescence microscope (Nikon, Japan).
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