Enhanced chemiluminescence ecl system kit

Dulbecco's modified eagle medium (DMEM), RPMI-1640 medium, fetal bovine serum (FBS), phenylmethylsulfonyl fluoride (PMSF); 3–4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI) and 5-fluorouracil (5-FU) was obtained from Sigma-Aldrich Co. LLC (ST. Louis, Mo, USA). Anti-caspase-3, anti-Bcl2, anti-Bcl-XL, anti-GAPDH, and rabbit anti-mouse horseradish peroxidase (HRP)-labeled secondary antibodies were procured from Cell Signaling Technology. Enhanced chemiluminescence (ECL) system kit, sterile filters (0.22 μm), and polyvinylidene difluoride (PVDF) membranes were procured from Millipore (MA, USA). Vybrant Apoptosis Assay Kit #2 was obtained from Molecular Probe (Eugene, Oregon, 97402-0469). Cell adhesion and cell migration kits were procured from Cell Biolabs, Inc.

AgNO₃ (CAS No. 7761-88-8), NaBH4 (CAS No.16940-66-2), N-acetyl-L-cysteine (NAC), 2, 7-dichlorofluorescein diacetate (CAS No. 4091-99-0), 5-bromo-2-deoxyuridine (BrdU; CAS No. 59-14-3) and Dulbecco’s Minimal Essential Medium (DMEM; CAS No. D5030-10L) were procured from Sigma Aldrich, USA. cDNA Synthesis kit was purchased from Fermentas, USA. Alexa 488-labelled antibodies were obtained from Molecular Probes. The Cell Titer-Glo Luminescent cell viability assay kit was procured from Promega Corp. (Madison, WI, USA). Anti-Atg5, Anti-Beclin1, anti-LC3B, Anti-pAMPK1α, Anti-GAPDH, Anti-β-actin, and rabbit anti-mouse horseradish peroxidase (HRP)-labeled secondary antibodies were procured from Cell Signaling Technology. Enhanced chemiluminescence (ECL) system kit, sterile filters (0.22 μm), and polyvinylidene difluoride (PVDF) membranes were procured from Millipore (MA, USA). DNA fragmentation ELISA kit was procured from Roche Diagnostics, Mannheim, Germany. All the chemicals used were of the highest purity grade. All the plastic wares used in the study for cell culture were procured from Thermo Fisher Scientific, USA.