L929 cell line was purchased from Korean Cell Line Bank, Seoul, Korea. The cells were seeded in 24-well plates (2 × 104 cells/well) and pre-incubated in growth medium for 24 hours (n = 4). After overnight attachment, cells were treated with the prepared extracts of sealers for 1, 3, 7, and 14 days. Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Briefly, 200 µL of MTT solution (0.5 mg/mL in phosphate buffered saline) (Amresco, Solon, OH, USA) was added to each well, and the wells were incubated for 2 hours. Subsequently, 200 µL of dimethyl sulfoxide (Amresco) was added to each well. Reduced MTT was then measured spectrophotometrically at 540 nm in a dual-beam microtiter plate reader (SPECTROstar Nano, BMG Labtech, Ortenberg, Germany).
L929 cell line
Manufactured by Korean Cell Line Bank
The L929 cell line is a type of mouse fibroblast cell line. It is commonly used in cell culture and biological research applications.
Automatically generated - may contain errors
2 protocols using l929 cell line
1
Cytotoxicity Evaluation of Dental Sealers
2
BALB/c Murine Bone Marrow-Derived Dendritic Cell Protocol
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All female BALB/c (6-week-old) mice were obtained from Orient Bio (Seongnam, Korea) and housed under pathogen-free conditions. All experiments were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of Sungkyunkwan University School of Medicine (approval number: SKKUIACUC2023-02-16-1). This university is accredited by the guidelines of the National Research Council’s Guide for the Care and Use of Laboratory Animals. The L929 cell line (mouse fibroblast of connective tissue) was obtained from Korean Cell Line Bank (Seoul, South Korea). Bone-marrow-derived DCs (BMDCs) were used for in vitro tests. BMDCs were prepared from murine bone marrow cell cultured with GM-CSF (20 ng/ml) for differentiation. 4T1-Luc2 (a luciferase-expressing cells derived from the parent 4T1 cell line) cells were purchased from American Type Culture Collection. These cells were cultured in RPMI 1640 medium (RPMI 1640 is supplemented with 10% FBS, 1% penicillin/streptomycin, and 1% l -glutamine).
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