MSCPat cells (MSC7-BJ), human primary BMSCs, derived from bone marrow aspirates previously described in (23 (link)) and hMPCs described in (24 (link)) were cultured in αMEM supplemented with 10% MSC-FBS (12662029; Life Technologies), Glutamine 10mM (Life Technologies) and 2 ng/mL Recombinant Human FGF (233-FB-025; R&D Systems). For these primary cells, written informed consent was obtained according to the Declaration of Helsinki and studies were approved by the ethics committees of the contributing institutions. A673 were cultured in DMEM medium supplemented with 10% FBS (Life Technologies) and cultured at 37 °C in a humidified atmosphere with 5% CO2 and 20% O2. MSCPat and hMPCs were cultured in hypoxic-like conditions (3% O2). Culture cells were tested monthly for mycoplasma contamination (with the VenorGeM qEP kit (11-9250, Minerva Biolabs), and if positive, cells were treated with the mycoplasma treatment kit (Myco-1&2 set A8360.0010, VWR). MSCPat were culture at low passages (up to 10). EWIma clones were kept in culture from passage 1 and up to 100 days in culture. Cells used in the study are fully described in Supplementary Materials .
Recombinant human fgf
Manufactured by R&D Systems
Recombinant Human FGF is a laboratory product that contains the Fibroblast Growth Factor (FGF) protein, which is produced through recombinant DNA technology. FGF is a signaling protein involved in various biological processes, including cell growth, differentiation, and angiogenesis. This product can be used for research purposes in the study of FGF and its functions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Venorgem qep kit, by Minerva Biolabs (1 mentions)
Msc fbs, by Thermo Fisher Scientific (1 mentions)
Recombinant human leukemia inhibitory factor, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dispase 2, by Merck Group (1 mentions)
α minimal essential medium, by Thermo Fisher Scientific (1 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Merck Group (1 mentions)
2 protocols using recombinant human fgf
1
Cultivation of Mesenchymal Stem Cells from Diverse Sources
2
Isolation and Culture of Mouse Adipose Stem Cells
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ASCs were isolated from mouse subcutaneous, abdominal, and perivascular adipose tissue, as described previously (Gu et al., 2019 (link)). Briefly, adipose tissue was cut into 1-mm3 small pieces, which was then digested with 2 mg/mL collagenase type I (Gibco) and 1 mg/mL Dispase II (Sigma) in Hanks’ balanced salt solution (HBSS) at 37°C for 30–45 min with occasional vortex. The digestion was stopped by DMEM/F12 with 10% FBS and subsequently passed through a 100-μm filter followed by a centrifugation at 300 g for 5 min. Cell precipitates were resuspended in a stem cell culture medium as the following: Minimal Essential Medium-α (Gibco) with 15% fetal bovine serum (Gibco), 10 ng/mL recombinant human leukemia inhibitory factor (Sigma), 5 ng/mL recombinant human FGF (R&D), 2 mmol/L L-glutamine (Sigma), 100 U/mL penicillin, and 100 mg/mL streptomycin (Gibco); they were then incubated in a 5% CO2 incubator. The cells were passaged at a ratio of 1:3 every 2 or 3 days. The medium was refreshed every 2 days.
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