MDA-MB-231 and MTV/TM-011 cells (1 × 105 cells) were suspended in serum-free medium containing DMSO or Stattic and seeded into the upper transwell inserts (Corning Inc., Cat#. 354480). The lower chambers were filled with medium containing 20% FBS. After incubation for 30 h, the bottom of transwell inserts was fixed with cold methanol and stained with 0.5% crystal violet. The non-invaded cells were wiped off and the invaded cells were counted in four randomly selected fields using an inverted microscope (100× magnification).
Upper transwell inserts
Manufactured by Corning
The Upper Transwell Inserts are a laboratory equipment used for cell culture experiments. They provide a porous membrane support that allows for the study of cellular interactions and permeability across a barrier. The inserts are designed to fit into a multi-well plate and facilitate the separation of upper and lower compartments for controlled experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using upper transwell inserts
1
Transwell Invasion Assay for Cell Migration
2
Cell Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTV/TM-011 cells (1 × 105 cells) were suspended in serum-free medium containing each compound and seeded into the upper transwell inserts (Corning Inc., Cat#. 354480). The lower chambers were filled with medium containing 20% FBS. After incubation for 30 h, the bottom of transwell inserts was fixed with cold methanol and stained with 0.5% Crystal Violet. The non-invaded cells were wiped off and the invaded cells were counted in four randomly selected fields using an inverted microscope (100× total magnification).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!