The liver tissues were collected with the RIPA buffer (R0020, Solarbio, Beijing, China). The protein contents were detected by a Bicinchoninic acid (BCA) protein assay kit (P0010, Beyotime, Shanghai, China). Equal protein amounts were loaded for protein electrophoresis. The antibodies for proteins were purchased from Proteintech (Wuhan, Hubei, China): TLR4 (1:1000, 66350-1-Ig); IL-1β (1:1000, 168-1-AP); SPHK2 (1:3000, 17096-1-AP); BCL2 (1:1000, 66799-1-lg); NF-κB (1:2000, 10745-1-AP); and GAPDH (1:100,000, 60004-1-lg). The IgG-horseradish peroxidase (HRP) secondary antibodies (1:10,000) were incubated. Protein bands were exposed using a Novex™ ECL Chemiluminescent Kit (WP20005, Thermo Fisher Scientific, Waltham, MA, USA).
Novex ecl chemiluminescent kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Novex™ ECL Chemiluminescent Kit is a laboratory equipment product designed for the detection and visualization of proteins in Western blot analysis. The kit provides the necessary reagents to enable chemiluminescent detection of target proteins, allowing for sensitive and accurate visualization of protein bands on a membrane.
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Lab products found in correlation
2 protocols using novex ecl chemiluminescent kit
1
Liver Tissue Protein Expression Analysis
2
Liver Tissue Protein Expression Analysis
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The liver tissues were collected with the RIPA buffer (R0020, Solarbio, Beijing, China). The protein contents were detected by a Bicinchoninic acid (BCA) protein assay kit (P0010, Beyotime, Shanghai, China). Equal protein amounts were loaded for protein electrophoresis. The antibodies for proteins were purchased from Proteintech (Wuhan, Hubei, China): TLR4 (1:1000, 66350-1-Ig); IL-1β (1:1000, 168-1-AP); SPHK2 (1:3000, 17096-1-AP); BCL2 (1:1000, 66799-1-lg); NF-κB (1:2000, 10745-1-AP); and GAPDH (1:100,000, 60004-1-lg). The IgG-horseradish peroxidase (HRP) secondary antibodies (1:10,000) were incubated. Protein bands were exposed using a Novex™ ECL Chemiluminescent Kit (WP20005, Thermo Fisher Scientific, Waltham, MA, USA).
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