Glucose concentration was analyzed in the supernatant using high-performance liquid chromatography (HPLC). A Waters Acquity UPLC® H-Class System (Waters, Zellik, Belgium) with an ion-exchange Aminex HPX-87H column 7.8 × 300 mm (Bio-Rad Laboratories N.V., Temse, Belgium) heated up to 50 °C was used for analysis. A metabolite analysis was carried out with an isocratic flow rate of 0.6 mL min−1 for 25 min. The mobile phase was composed of water containing 5 mM H2SO4. Elution profiles were monitored through a Waters Acquity® Refractive Index Detector (RID) (Waters, Zellik, Belgium). A glucose standard solution (Sigma-Aldrich, Overijse, Belgium) was used to determine the retention time and to establish a calibration curve.
Waters acquity refractive index detector
Manufactured by Waters Corporation
Sourced in Belgium
The Waters Acquity® Refractive Index Detector (RID) is a laboratory instrument used to detect and quantify analytes based on their refractive index. It is designed to provide reliable and precise measurements for a wide range of applications.
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Lab products found in correlation
2 protocols using waters acquity refractive index detector
1
Glucose Quantification by HPLC
2
Supernatant Glucose and Arabinose Analysis
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Supernatant glucose and arabinose concentrations were analyzed by high-performance liquid chromatography (Waters Acquity UPLC® H-Class System) using an ion-exchange Aminex HPX-87H column (7.8 × 300 mm, Bio-Rad Laboratories N.V.). The analysis was carried out with an isocratic flow rate of 0.6 mL min -1 for 25 min at 50°C. The mobile phase was composed of an aqueous solution of 5 mM H2SO4. Elution profiles were monitored through a Waters Acquity® Refractive Index Detector (RID) (Waters, Zellik, Belgium). Glucose and arabinose standard solutions (Sigma-Aldrich, Overijse, Belgium) were used to determine the retention times and construct calibration curves.
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