All 190 S. aureus isolates were analyzed at OSU Wexner Medical Center using MALDI-TOF, Biotyper 2.0, Bruker, following the manufacturer’s protocol. Antimicrobial susceptibility test (AST) was performed using VITEK® 2 XL (bioMérieux version 08.01) and the result was interpreted following minimum inhibitory concentration (MIC) interpretation guide of Clinical Laboratory Standards Institution (CLSI) M100-S22 (2018). Based on the result of cefoxitin test, isolates were grouped into MRSA or MSSA.19
Biotyper 2
Manufactured by Bruker
Sourced in Germany
The Biotyper 2.0 is a MALDI-TOF mass spectrometry-based system designed for the rapid identification of microorganisms. It provides a fast and reliable method for the identification of bacteria, yeasts, and fungi from clinical and environmental samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using biotyper 2
1
MALDI-TOF and VITEK 2 Antibiotic Susceptibility of S. aureus
2
Vibrio cholerae Serotyping Protocol
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Identification of the isolates at species level was confirmed by MALDI-TOF MS using Biotyper 2.0 (Bruker Daltonics GmbH, Bremen, Germany) [11 (link)]. Serogroup and serotype were confirmed using the Vibrio cholerae E Agglutinating Sera kit containing specific antisera O1 polyvalent agglutination serum, Inaba agglutination serum, and Ogawa agglutination serum (Remel Europe Ltd. Darford, Kent, United Kingdom) according to the manufacturer’s guidelines.
3
MALDI-TOF MS for Microbial Identification
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Thawed clinical isolates were plated on blood agar plates and after 24 hours of incubation at 37℃, a subculture was made to another blood agar plate (Asan Pharmaceutical, Seoul, Korea). The cells were extracted using a formic acid/acetonitrile method, as described previously.11 (link) One microliter of each extract was spotted onto a sample target, overlaid with an HCCA (acyano-4-hydroxycinnamic acid) matrix solution, and loaded into BIOTYPER 2.0 (Bruker Daltonics, Bremen, Germany).
The obtained spectra profiles were compared with a database (V2.0.4.0) containing reference spectra of roughly 1900 microbial species. The peak intensities for each isolate were associated with the respective classes. Based on these mass classes, a similarity matrix was calculated to compare the results of the MALDI-TOF MS with the results of the other identification methods
The obtained spectra profiles were compared with a database (V2.0.4.0) containing reference spectra of roughly 1900 microbial species. The peak intensities for each isolate were associated with the respective classes. Based on these mass classes, a similarity matrix was calculated to compare the results of the MALDI-TOF MS with the results of the other identification methods
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