L proline

This S.chaetoloma specimen was acquired in Suiyang, Guizhou, China, in October 2021. Amentoflavone (AME), robustaflavone (ROB), hinokiflavone (HIN), and heveaflavone (HEV) were acquired from Ruifensi Biotechnology Co., Ltd (Sichuan, China). Choline chloride, betaine, tetrapropylaminium bromide, tetraethylammonium bromide, tetramethylammonium bromide, 1,1-Dipheny-2-picrylhydrazyl (DPPH), 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), 1,2,4-Butanetriol, geraniol, DL-Malic acid, lactic acid, maltose, citric acid, D-Sorbitol, 1-Nonanol, glycerol, terpinen-4-ol, erythritol, 1,4-Butanediol, D-Mannose, L-Proline, urea, xylitol, nerolidol, alpha-glucosidase (AG), p-Nitrophenol (PNP), and p-Nitrophenyl α-D-glucopyranoside (pNPG) were purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China). A549 (human lung cancer), HCT-116 (human colon cancer), HT-29 (human colon cancer) and HepG2 (human liver cancer) cells were acquired from Yu chun Biotechnology Co., Ltd (Shanghai, China).

l-Lysine, l-histidine, l-aspartate, l-cysteine, l-tryptophan, l-serine, l-phenylalanine, l-glycine, l-valine, l-alanine, l-leucine, l-isoleucine, l-threonine, l-methionine, l-proline, and l-glutamic acid were purchased from Yuanye Biological Technology Co., Ltd. (Shanghai, China; purity >99.5%). Copper sulfate and sodium acetate were purchased from Shuang Shuang Chemical Reagent Co., Ltd (Yantai, China). The purified water used to prepare various solutions was obtained from the GLP Laboratory of Lanzhou University (Lanzhou, China). Acetic acid (HPLC grade) was purchased from Shandong Yu Wang Pharmaceutical Co., Ltd. (Shandong, China). Three kinds of beers were purchased from a local supermarket (Lanzhou, China).

Tan IIA (purity > 98%), [U-¹³C]-glucose, l-serine, l-glycine, l-lactic acid, l-alanine, pyruvate, l-malic acid, succinate, fumarate, l-glutamic acid, citric acid, α-ketoglutaric acid, l-asparatic acid, and l-proline were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Recombinant human TGF-β1, Yefluor 488 EdU imaging kit, and cell cycle and apoptosis analysis kit were purchased from Yeasen (Shanghai, China). Sulforaphane (SFN), dimethyl sulfoxide (DMSO) and methoxyamine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). d-glucose was purchased from Aladdin (Shanghai, China). Dulbecco's modified Eagle medium (DMEM), minimum essential medium (MEM), and 0.25% trypsin were purchased from Gibco (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) was purchased from Vazyme (Nanjing, China). SB 204990 was purchased from MedChemExpress (Princeton, NJ, USA). BTA was purchased from Macklin (Shanghai, China). Glucose assay kit was purchased from Elabscience (Wuhan, China). Lactate assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide was purchased from Acmec (Shanghai, China). Triton ×100, hoechst33342, antifade mounting medium and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime (Shanghai, China).