30 μM mouse serum albumin (MSA) was incubated with 30, 60 and 120 μM ABD-iTEP-sMMP-pOVA or ABD-iTEP-sMMP-pTRP2 at 4 °C for overnight. 100 μ L of the mixture was then taken to elute through the size exclusion column (diameter: 9.4 mm, length: 250 mm, particle diameter: 6.0 μm) equipped in an Agilent 1260 infinity liquid chromatography system (Agilent Technologies). The samples were eluted at a flow rate of 1 mL min−1 using 1× PBS. Absorbance spectra of each sample was monitored at 280 nm.
Agilent 1260 infinity liquid chromatography system
Manufactured by Agilent Technologies
The Agilent 1260 Infinity Liquid Chromatography System is a high-performance liquid chromatography (HPLC) platform. It is designed to provide reliable and efficient separation and analysis of complex samples. The system consists of various modules, including a pump, autosampler, column compartment, and detector, which work together to deliver accurate and reproducible results.
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2 protocols using agilent 1260 infinity liquid chromatography system
1
Size Exclusion Chromatography of MSA-ABD-iTEP Complexes
2
SEC Analysis of ABD-iTEP-MSA Complex
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SEC was conducted on an Agilent 1260 infinity liquid chromatography system (Agilent Technologies) equipped with an Agilent ZORBAX GF-450 size exclusion column (diameter: 9.4 mm, length: 250 mm, particle diameter: 6.0 µm). 60 µM ABD-iTEP was incubated with 30 µM MSA overnight at 4 °C. 100 µL sample was loaded for analysis. Samples were eluted with PBS at a flow rate of 1 mL/min. Spectra were monitored by absorption at 280 nm.
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