Anti human oct 4

Manufactured by Abcam

Sourced in United States

Anti-human OCT-4 is a laboratory reagent used to detect and quantify the expression of the OCT-4 protein in human samples. OCT-4 is a transcription factor that plays a crucial role in the maintenance of pluripotency in embryonic stem cells. This antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of OCT-4 in different cell types and tissues.

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Lab products found in correlation

Psicor, by Addgene (1 mentions) C myc, by Abcam (1 mentions) Anti human ssea4, by Abcam (1 mentions) Anti human abcg2, by Abcam (1 mentions) Anti human nanog, by Abcam (1 mentions) Anti human ki67, by Abcam (1 mentions)

2 protocols using anti human oct 4

Knockdown and Overexpression of Stem Cell Markers

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The shRNA plasmids for CD105 knockdown were constructed from pSicoR (Addgene, #11579) with target sequences of shENG1: GAA AGA GCT TGT TGC GCA T and shENG2: AAC AGT CCA TTG TGA CCT TCA. Data presented were from knockdown with shEGN1, which is consistent with the results from shENG2 (Figure S3). The ectopic overexpression plasmids of ENG, CDA, MYC, and NANOG were constructed based on the basic lentiviral vector.
Regarding antibodies, anti-human OCT-4 (#ab19857), SOX-2 (#ab171380), KLF4 (#ab72543), NANOG (#ab80892), and C-MYC (#ab32072) were bought from Abcam (MA, USA), anti-human ACTIN (#AC026) was bought from Abclonal (MA, USA), anti-human CD105 (#ab169545), CD44 (#ab51037), MUSASHI (#EP1302), NESTIN (#ab105389), and VIMENTIN (#ab16700) were bought from Epitomics (CA, USA), anti-human MHC-I (#GTX105052) and CK7(#GTX109723) were bought from Genetex (CA, USA), and anti-human DLK-1(#AP20959c) and CXCR4 (#AW5434-U080) were bought from Abgent (CA, USA). For flow cytometry, anti-human CD105 conjugated with PE antibody (#130-098-906) was bought from Miltenyi (CA, USA).

Hu J., Guan W., Liu P., Dai J., Tang K., Xiao H., Qian Y., Sharrow A.C., Ye Z., Wu L, & Xu H. (2017). Endoglin Is Essential for the Maintenance of Self-Renewal and Chemoresistance in Renal Cancer Stem Cells. Stem Cell Reports, 9(2), 464-477.

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Comprehensive Immunohistochemical Profiling of Corneal Cells

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For cell staining, cells on the coverslip were washed with PBS and fixed in 4% paraformaldehyde. For tissue staining, the whole cornea with limbus was carefully harvested and embedded in OCT compound. Eight-μm frozen sections were made and fixed in cold acetone, after which specimens were treated with 0.1% Triton X-100 for 30 min and blocked with 5% BSA for 1 h. Fluorescein conjugated or non-conjugated primary antibody at appropriate concentration was used for incubation at 4°C overnight. If necessary, specimens were then incubated with fluorescent second antibody at room temperature for 2 h. Finally, after stained with DAPI for 5 min, slides were mounted with mounting medium and observed with a fluorescence microscopy (Nikon, Tokyo, Japan). The primary antibodies used were as follows: anti-human p63, anti-human Ki67, anti-human ABCG2, anti-human CK3/12, anti-human ABCB5, anti-human SSEA4, anti-human Nanog, anti-human OCT4 (all from Abcam, Cambridge, MA), anti-rabbit CD11b, anti-rabbit CD161, anti-rabbit CD4, and anti-rabbit CD8 (all from Biolegend, San Diego, CA).

Wang Z., Zhou Q., Duan H., Wang Y., Dong M, & Shi W. (2016). Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells. PLoS ONE, 11(3), e0150731.

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