Syto rnaselect dyes

Manufactured by Thermo Fisher Scientific

SYTO RNASelect dyes are a series of fluorescent dyes designed for the specific labeling and detection of RNA in live cells. These dyes selectively bind to RNA and exhibit enhanced fluorescence upon binding, allowing for the visualization and quantification of RNA within a sample.

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Lab products found in correlation

Clathrin, by Abcam (1 mentions) Acridine orange, by ImmunoChemistry Technologies (1 mentions) Grp78, by Cell Signaling Technology (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Vinculin, by Merck Group (1 mentions) Lysotracker, by Thermo Fisher Scientific (1 mentions) Nestin, by R&D Systems (1 mentions) Bafilomycin a1 bafa1 sc 201550, by Santa Cruz Biotechnology (1 mentions) Pou3f2, by Cell Signaling Technology (1 mentions) Olig2, by Merck Group (1 mentions) V atpase g1, by Proteintech (1 mentions) Hoxa7, by Abcam (1 mentions) V atpase g2, by Proteintech (1 mentions) Calnexin, by Abcam (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Nh4cl, by Merck Group (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Annexin 5, by BD (1 mentions) Tsg101, by Proteintech (1 mentions) Propidium iodide, by BioLegend (1 mentions)

Spelling variants of this product

SYTO RNASelect (40 citations)

2 protocols using syto rnaselect dyes

Multiparametric Analysis of Cell Cultures

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Lysotracker, CellTrace Violet, FM lipophilic styryl (FM 1-43FX) and SYTO RNASelect dyes were from Life Technologies (Thermo Fisher Scientific). Acridine Orange was from ImmunoChemistry Technologies (Bloomington, MN, USA). PropidiumIodure (PI) and Annexin V were from BD Bioscience (FITC-Annexin V Apoptosis Detection Kit). Bafilomycin A1 (BafA1; sc-201550) was from Santa Cruz Biotechnologies whereas NH4Cl (254134) was from Sigma Aldrich.
The following primary antibodies were used for immunofluorescence, immunoblotting (IB) and flow cytometry (FACS) assays: V-ATPase G1 (16143-1-AP, Proteintech), Vinculin (V9131, Sigma Aldrich), Tsg101 (14497-1-AP, Proteintech), Ago2 (10686-1-AP, Proteintech), Clathrin (ab23440, Abcam), CD63 (sc-15363, Santa Cruz), CD9 (IB: 10626D, Thermo Fisher; FACS: 130-103-988, Miltenyi), CD81 (130-107-982, Miltenyi), Calnexin (ab31290, Abcam), Nestin (MAB 1259, R&D), Tuji (T3952, Sigma Aldrich), GFAP (G9269, Sigma Aldrich), CD11b (20991-1-AP, Proteintech), O4 (O7139, Sigma Aldrich), Olig2 (AV32753, Sigma Aldrich), CD31 (ab28364, Abcam), Vimentin (130-106-369, Miltenyi), POU3F2 (12137, Cell Signaling), HOXA10 (TA590263, Origen), HOXA7 (ab211521, Abcam), V-ATPase G2 (25316-1-AP, Proteintech), ARF6 (6ARF01, ThermoFisher Scientific), and GRP78 (3177, Cell Signaling).

Bertolini I., Terrasi A., Martelli C., Gaudioso G., Di Cristofori A., Storaci A.M., Formica M., Braidotti P., Todoerti K., Ferrero S., Caroli M., Ottobrini L., Vaccari T, & Vaira V. (2019). A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes. EBioMedicine, 41, 225-235.

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Isolation and Characterization of Group A Streptococcus Membrane Vesicles

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MVs were routinely isolated from late-logarithmic- or early-stationary-phase GAS cultures in THB. Bacteria were pelleted at 2,500 × g, and the supernatant was decanted and filtered twice through 0.22-µm-pore polyethylsulfone membranes prior to ultracentrifugation (175,000 × g, 4 h, 4°C). Pellets obtained after ultracentrifugation were directly quantified or were washed once in phosphate-buffered saline (PBS) and further processed for other analyses (described below). MVs were quantified by Bradford protein determination assay (Sigma) or by incubation with 0.5 µg/ml of lipophilic membrane dye FM1-43 (Life Technologies) for 15 min, prior to flow cytometric counting using Count Bright counting particles (Life Technologies). MV production was normalized to the CFU of each strain. For detection of MV RNA species, MVs and GAS were incubated with Benzonase (see the protocol for vesicular RNA isolation below) and stained with both 0.5 µg/ml of FM4-64 and 1 µM Syto RNAselect dyes (Life Technologies) or with propidium iodide (BioLegend) for 15 min prior to detection by flow cytometry.

Resch U., Tsatsaronis J.A., Le Rhun A., Stübiger G., Rohde M., Kasvandik S., Holzmeister S., Tinnefeld P., Wai S.N, & Charpentier E. (2016). A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus. mBio, 7(6), e00207-16.

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