96 well tissue plates

Manufactured by Greiner

Sourced in Germany

The 96-well tissue plates are a laboratory equipment designed for cell culture applications. They provide a standardized multi-well format for seeding and growing cells. The plates have a flat bottom and are made of tissue culture-treated polystyrene to support cell attachment and proliferation.

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Lab products found in correlation

Fetal calf serum (fcs), by Thermo Fisher Scientific (3 mentions) Celltiter 96 kit, by Promega (2 mentions) Celltiter 96, by Promega (1 mentions)

Close spelling variants of this product

U-bottom 96-well tissue culture plates 96-well flat-bottom tissue culture plates 96-well luminometer-compatible tissue culture plates CELLSTAR μClear® 96-well tissue culture plates Cellstar 96-well tissue culture plates 96-well tissue culture treated plates 96-well flat-bottom tissue plates 96-well plates

3 protocols using 96 well tissue plates

Galectin-3 Inhibition Assay on Neuroblastoma

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Human neuroblastoma cells (strain SK-NM-C) were grown in 96-well tissue plates (Greiner, Nürtingen, Germany) at 37 °C for 48 h in Eagle’s minimal essential medium with 10% fetal calf serum (Thermo Fisher Scientific, Dreieich, Germany) and non-essential amino acids in the absence (control) and in the presence of 125 µg/mL galectin (in four independent experimental series run in triplicate per protein from aliquots of the same cell suspension per assay series). The cell count was determined using the reagents of the CellTiter 96 kit (Promega), and the data obtained for the controls and the WT and variant proteins were statistically processed by t tests, as described previously (Kopitz et al. 2003 (link), 2017 (link); Ruiz et al. 2014 (link)). Competition assays with WT Gal-3 as inhibitor were performed by adding this protein at a 10:1 ratio to solutions containing test galectins, as performed previously (Kopitz et al. 2001 (link), 2003 (link)).

Ludwig A.K., Michalak M., Gabba A., Kutzner T.J., Beckwith D.M., FitzGerald F.G., García Caballero G., Manning J.C., Kriegsmann M., Kaltner H., Murphy P.V., Cudic M., Kopitz J, & Gabius H.J. (2021). Imitating evolution’s tinkering by protein engineering reveals extension of human galectin-7 activity. Histochemistry and Cell Biology, 156(3), 253-272.

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Galectin-7 Binding and Proliferation in Neuroblastoma

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Neuroblastoma cells (strain SK-N-MC) were cultured at 37°C in Eagle'sminimal essential medium with 10% fetal calf serum (Thermo Fischer Scientific, Dreieich, Germany) and non-essential amino acids in an atmosphere of 95% air and 5% CO₂. Cells grown to confluency in 96-well tissue culture plates for five days (∼10⁵cells/well) were incubated for 16 h in medium without fetal calf serum. For the binding assays, serum-free Eagle's minimal essential medium (100 μl/well) with 25 mm HEPES, pH 7.4, 0.01% bovine serum albumin was used to block protein-binding sites, and the labeled galectins (specific radioactivities (150 kBq/µg for galectin-7 and 144 kBq/µg) at non-saturating concentrations. Protein iodination, measurement of carbohydrate-inhibitable binding of ¹²⁵I-labelled Gal-7 proteins to the cultured cells and data processing followed an optimized procedure [46 (link)].
Cell proliferation in the presence or absence of a Gal-7 protein was examined in 96-well tissue plates (Greiner, Nürtingen, Germany) by culturing cells in 100 µl medium for 48 h at 37°C and measuring cell growth with a cell proliferation kit (CellTiter-96, Promega, Heidelberg, Germany), as described previously [47 (link),48 (link)]. The effect of the presence of 25 µg cholera toxin B-subunit (Ctx-B; Sigma) was examined after co-incubation with the tested galectin [47 (link),48 (link)].

Miller M.C., Nesmelova I.V., Daragan V.A., Ippel H., Michalak M., Dregni A., Kaltner H., Kopitz J., Gabius H.J, & Mayo K.H. (2020). Pro4 prolyl peptide bond isomerization in human galectin-7 modulates the monomer-dimer equilibrum to affect function. Biochemical Journal, 477(17), 3147-3165.

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Human Neuroblastoma Cell Proliferation Assay

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Human neuroblastoma cells (strain SK-NM-C) were grown in 96-well tissue plates (Greiner, Nürtingen, Germany) at 37 °C for 48 h in Eagle’s minimal essential medium with 10% fetal calf serum (Thermo Fisher Scientific, Dreieich, Germany) and non-essential amino acids in the absence (control) and in the presence of 125 μg/mL galectin (in four independent experimental series run in triplicate per protein from aliquots of the same cell suspension per assay series). The cell count was determined using the reagents of the CellTiter 96 kit (Promega), and the data obtained for the controls and the WT and variant proteins were statistically processed by t tests, as described previously (Kopitz et al. 2003 (link), 2017 (link); Ruiz et al. 2014 (link)). Competition assays with WT Gal-3 as inhibitor were performed by adding this protein at a 10:1 ratio to solutions containing test galectins, as performed previously (Kopitz et al. 2001 (link), 2003 (link)).

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