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Goat anti rabbit texas red t2767

Manufactured by Thermo Fisher Scientific
Sourced in United States

Goat anti-rabbit Texas-red (T2767) is a secondary antibody produced in goats and conjugated with the Texas Red fluorescent dye. It is designed to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications.

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2 protocols using goat anti rabbit texas red t2767

1

Fluorescence Immunohistochemistry Protocol

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The primary antibodies used in fluorescence immunohistochemistry were obtained from commercial sources: anti-WT-1 (ab15249)(Abcam, Cambridge, UK) rabbit polyclonal primary antibody; anti-E-cadherin (13-1900)(Thermo Fisher Scientific, Waltham USA) rat monoclonal antibody; anti-Vimentin (ab24525)(Abcam) chicken polyclonal primary antibody; and anti-Mesothelin (250519)(Abbiotec, Escondido, CA USA) rabbit polyclonal antibody. The cross-absorbed detection (secondary) antibodies were also obtained from commercial sources: goat anti-rabbit Texas-red (T2767)(Thermo Fisher); goat anti-chicken FITC (PA1-28794)(Thermo Fisher); and goat anti-rat Texas-red (1010-07)(Southern Biotechnology, Birmingham AL, USA). Primary antibodies were used at a 5-fold saturating concentration. Secondary antibodies were titered to minimize nonspecific background staining; negative controls were stained with detection antibody alone.
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2

Fluorescence Immunohistochemistry Protocol

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The primary antibodies used in fluorescence immunohistochemistry were obtained from commercial sources: anti-WT-1 (ab15249)(Abcam, Cambridge, UK) rabbit polyclonal primary antibody; anti-E-cadherin (13-1900)(Thermo Fisher Scientific, Waltham USA) rat monoclonal antibody; anti-Vimentin (ab24525)(Abcam) chicken polyclonal primary antibody; and anti-Mesothelin (250519)(Abbiotec, Escondido, CA USA) rabbit polyclonal antibody. The cross-absorbed detection (secondary) antibodies were also obtained from commercial sources: goat anti-rabbit Texas-red (T2767)(Thermo Fisher); goat anti-chicken FITC (PA1-28794)(Thermo Fisher); and goat anti-rat Texas-red (1010-07)(Southern Biotechnology, Birmingham AL, USA). Primary antibodies were used at a 5-fold saturating concentration. Secondary antibodies were titered to minimize nonspecific background staining; negative controls were stained with detection antibody alone.
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