Cd138 microbeads

Patients and healthy volunteers were informed to sign the informed consent forms before sample collection. The study has been approved by the Ethics Committee of Shanghai Jiao-Tong University School of Medicine. Mononuclear cells (MNCs) were isolated from the bone marrow specimens using Stemcell Lymphoprep^TM (Stemcell). CD138⁺ cells of the active MM patients were obtained from the bone marrow samples using CD138⁺ microbeads (Stemcell).

The HMCLs ARP-1 and U266 were generously provided by Dr. Qing Yi (Center for Hematologic Malignancy, Research Institute, Houston Methodist, Houston, TX, USA), and RPMI-8226 and MM1.S were purchased from the National Collection of Authenticated Cell Cultures (Beijing, China). All cell lines were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS, Biological Industries, Israel) at 37 °C under 5% CO₂ atmosphere.
With the approval of the Ethics Committee of the First Affiliated Hospital of Zhejiang University School of Medicine and informed consents obtained from MM patients, we fetched bone marrow samples for the experimental study only. Primary MM cells were sorted by CD138 microbeads(STEM CELL Technologies, Canada), and then cultured in RPMI-1640 medium containing 20% FBS at 37 °C under 5% CO₂ atmosphere.
Chidamide and venetoclax were purchased from Med Chem Express (New Jersey, USA).