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Fv 10 viewer

Manufactured by Olympus

The FV-10 viewer is a microscope eyepiece that allows users to view and examine samples under magnification. It provides a clear and focused visual representation of the sample, enabling detailed observation and analysis.

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Lab products found in correlation

2 protocols using fv 10 viewer

1

Quantifying GCL Volume and Cellular Proliferation

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6–7 confocal z-stack images per animal were quantified (N = 4 in every group). The volume of the GCL was calculated by measuring the GCL area in the image (using Olympus FV-10 viewer) and multiplying the measured area by the number of z-axis slices and the distance between slices (1.46 µm). Cells within the GCL (Ki67/CldU/GFP) were counted manually by a blind experimenter (using Olympus FV-10 viewer). Z-plane projections used to verify double labeling. Student’s T-test (SPSS) was used to compare between groups, assuming a normal distribution and unequal variance.
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2

Anterior Eye Tissue Imaging

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The anterior eye, including the cornea and TM, was dissected and incubated with DAPI for 30 minutes. After thoroughly washing with PBS, the tissues were flat-mounted and imaged on a confocal microscope (Olympus) with a movable stage for stitched stacks and analyzed using FV10 Viewer.
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